血吸虫色素类似物(β-hematin)体外形成系统的初步研究

Primary Study of Schistosoma Pigment Analog(β-hematin) Formation System in vitro

为了建立血吸虫色素体外形成系统来筛选阻断血吸虫色素形成的药物,利用分光光度法分析了血吸虫色素、pH、NaAc浓度、KAc浓度、反应时间、还原剂β-巯基乙醇对-βHematin形成的影响,并利用色素形成抑制剂氯喹验证其有效性.结果表明,在0.5 mol/L醋酸钠系统中,低pH值(小于5)、高Na+或K+离子浓度、添加血吸虫色素以及延长反应时间均有利于β-Hematin的形成.而还原剂β-巯基乙醇可完全阻断色素形成.另外,对色素形成有明确抑制作用的氯喹可在一定范围内抑制β-Hematin的形成.因此,以"NaAc-Hematin"或"KAc-Hematin"系统为基础,辅之以低pH、高离子浓度以及适宜的反应时间等,可建立有效的血吸虫色素体外形成系统.

To establish the system of schistosoma pigment formation in vitro to screen the pigment formation inhibitors, the effect of influencing factors, including schistosoma pigment, pH, Na^＋ or K^＋ concentration, incubation time, reducing agent and chloroquine on β-hematin formation were detected using spectrophotometry. The results show that low pH, higher Na^＋ or K^＋ concentration, prolonged incubation time, adding schistosoma pigment all can promote β-hernatin formation. On the contrary, β-Mercaptoethamol as a reducing agent, strongly inhibit the process. Therefore, we propose a system of schistosoma pigment formation in vitro with low pH（ 〈 5） and high ion concentration（ 1-2 mol/L） based on ＂NaAc-hematin＂ system or ＂KAc-hematin＂ system, which has been testified to be effective for screening the pigment formarion inhibitors.