益寿饮颗粒对衰老动物模型免疫功能的影响

Effect of Immunological Function by Yishouyin Granula in Aging Animal Models

目的探讨益寿饮颗粒剂对衰老动物模型免疫功能的影响。方法模型大、小鼠各60只,按20只分组,分为生理盐水对照组、益寿饮小剂量组和益寿饮大剂量组。各组连续灌胃30d,停药后小鼠摘取眼球取肝素抗凝静脉血,大鼠自尾静脉取血,测IL-1α,IL-2,IL-6,RBC-C3bRR,RBC-ICR以及外周血T淋巴细胞亚群。结果大、小剂量组与对照组比较,IL-1α,IL-2,IL-6水平明显升高,RBC-C3bRR结合率明显升高,RBC-ICR结合率明显下降,CD3+,CD4+淋巴细胞的比值明显升高,CD8+淋巴细胞的比值明显降低,各项指标均具有统计学意义。结论益寿饮颗粒提高衰老动物模型T淋巴细胞增殖和产生白介素-1(IL-1)、白介素-2(IL-2)、白介素-6(IL-6)的能力,增强红细胞膜RBC-C3bRR活性及粘附IC的能力,影响外周血T淋巴细胞亚群分类的水平,影响外周血T淋巴细胞亚群的数量、活性及亚群细胞之间的比例,调节T细胞的免疫功能,改善机体免疫调节功能。

Objective To investgate the effect of immunological function by Yishouyin granula in aging animal models. Methods Sixty Wistar rats were divided into 3 groups：low dosage group, high dosage group and control group. After 30 day ＇s ingastrio - infusion, the level of serum IL - 1 α, IL - 2, IL - 6, RBC - C3 bRR, RBC - ICR and T lymmphcyte sub groups were determined by enzymyradiography. Results Compared to control group, the level of serum IL - 1 α, IL - 2, IL - 6 were higher significantly, the combinated rate of RBC - C3bRR was eleveted, the combinated rate of RBC - ICR was decreased significantly;the level of CD3 ^＋ CD4 ^＋ was eleveted,while CD8^＋ was decreased significantly. Conclusion It was drawn from the experiment that Yishouyin granula could better the immunological function by eleveting the level of serum IL - 1 α, IL - 2, IL - 6 and strongthening the active function of RBC - Cs bRR as the same as adherion of immunological complex.