胶质母细胞瘤染色体1p36杂合性缺失的初步研究

A Primary Study of Loss of Heterozygosity on Chromosome 1p36 in Glioblastomas

目的探讨1p36上可能存在的与胶质母细胞瘤(GBM)发生有关的肿瘤抑制基因的缺失区域,为发现和定位肿瘤抑制基因提供线索和依据。方法运用聚合酶链反应(PCR)技术为基础的杂合性缺失分析(LOH)法对12例胶质母细胞瘤染色体1p36上12个微卫星多态标记序列作了较详细研究。结果12例胶质母细胞瘤至少有一个位点出现杂合性缺失者11例,总缺失率为91.7%(11/12),其中以D1S214-D1S2666位点间区域LOH的频率最高。结论胶质母细胞瘤染色体1p36发生高频率杂合性缺失,提示位点D1S214-D1S2666缺失区域间可能存在着与GBM发生有关的抑癌基因。

Objective The study of loss of heterozygosity（LOH） was performed to locate the deletion areas probably harboring tumor suppressor genes and provide clues for discovering tumor suppressor genes. Methods Twelve polymorphie mierosatellite markers on chromosome 1p36 in 12 eases of glioblastomas were analyzed to detect LOH by PCR based techniques. Results 91.7% informative eases of Glioblastomas displayed LOH on 1 p36. The most frequent LOH were observed at loci from D1S214 to D1S2666 on 1p36. Conclusion The highly frequent LOH was observed on chromosome 1 p36 in human Glioblastomas. The results of this study indicate that the chromosomal regions at loci from D1 S214 to D1 S2666 on 1p36 may contain tumor suppressor genes associated with the origin of Glioblastomas.