非霍奇金淋巴瘤患者血浆内皮生长因子水平测定及临床意义

The Clinical Significance of Detecting Plasma Vascular Endothelial Growthfactor Concentration in Non-hodgkin's Lymphoma

目的通过检测非霍奇金淋巴瘤（NHL）患者血浆中血管内皮生长因子（VEGF）水平，探讨其临床意义。方法应用双抗体夹心酶联免疫吸附测定法（ELISA）检测初治40例NHL患者和20例正常对照组血浆中VEGF含量。结果NHL患者VEGF水平明显高于对照组（P〈0．01），病变为Ⅲ～Ⅳ期、有纵隔浸润或腹部伴纵隔浸润患者血浆VEGF水平明显高于病变为Ⅰ～Ⅱ期、仅浅表淋巴结肿大患者（P〈0．01）。经治疗后达完全缓解的患者血浆VEGF水平下降至正常或接近正常，与治疗前相比，差异有显著性（P〈0．01），复发／进展者VEGF水平与治疗前相比，差异无显著性（P〉0．05）。结论血浆VEGF水平可反映体内淋巴瘤细胞的总负荷和疾病状态，测定血浆VEGF水平对NHL分期、病变部位的判断、疗效观察和预后具有一定临床意义。

Objective From detecting the VEGF levels in the plasma of NHL patients,to explore the clinical significance of the vascular endothelial growthfactor. Methods The plasma vascular endothelial growthfactor levels were detected in 40 cases of initial treatment non-Hodgkin＇s lymphoma patients and 20 normal control cases by double-antibody sandwich enzyme-linked immunosorbent assay way. Results Compared with normal control group the vascular endothelial growthfactor levels of non-Hodgkin＇s lymphoma patients were significantly higher（ P 〈0.01 ）. The plasma vascular endothelial growthfactor levels of the cases of Ⅲ - Ⅳstage or mediastinal infiltration invasion and abdomen with mediastinal or abdominal infiltration were significantly higher than the lesions of Ⅰ - Ⅲstage or only with superficial lymphadenectasis（ P 〈 0.01 ）. The vascular endothelial growthfactor levels of the complete remission patients were decreased to normal or near normat after treatment. There were significant differences compared with the levels before treatment（ P 〈 0.01 ）. There was no significant difference between recurrence or aggravation patients and the levels before treatment （ P 〉 0.05 ）. Conclusion The plasma vascular endothelial growthfactor levels can reflect the vivo lymphoma cells overall load and morbid state. There were important clinical significance of detecting the plasma vascular endothelial growthfactor levels in the staging of non-Hodgkin ＇s lymphoma, the judgment of diseased region, pathogenetic condition, the observation of therapeutic effect and prognosis.