犬急性重症胆管炎新模型建立

Establishment of a novel canine model of severe acute cholangitis

目的探讨建立一种适合于外科研究的犬急性重症胆管炎(ACST)模型的方法。方法将10只犬随机分为两组,造模组和对照组(假手术组)。造模组采用胆总管置入T管,远端结扎,T管内注入大肠埃希菌后夹闭T管。观察手术后1周内动物一般情况,分别于术前、术后24,48,72h和7d采血检测白细胞及分类、血清总胆红素(TB)、碱性磷酸酶(AKP)、谷丙转氨酶(ALT),TNF-α,IL-1β以及内毒素水平。处死实验动物观察胆管病理改变。结果术前两组之间各项指标差异无显著性(均为P>0.05)。术后造模组犬血清TB,AKP,ALT,TNF-α,IL-1β及内毒素水平逐渐上升,且均明显高于对照组(P<0.05)。造模组胆管病理表现为明显的急性炎症。结论该法建立犬重症胆管炎模型,可用于ACST的相关研究。

Objective To establish a novel canine model of severe acute cholangitis （ ACST）, Methods Ten dogs were randomly divided into two groups： model group and control group. ACST models were established by placing T-tube in common bile duct and ligating the distal bile duct, then injecting E. coli trough T-tube and closing the T-tube. The changes of peripheral WBC, total bilirubin, ALT, AKP, IL-1β, TNF-α and endotoxin were observed pre-operatively and at 24 h, 48 h, 72 h, and 7 d post-operatively. The pathological changes of bile duct were observed when the dogs were sacrificed. Results There were no significant differences in all indices between two groups pre-operatively. Serum IL-1β, TNF-α, endotoxin and total bilirubin, ALT, AKP level in model group increased gradually and were higher than control group （P 〈 0, 05 ） post-operatively. Marked acute inflammation of bile duct was observed in model group. Conclusions This method of establishing canine ACST model can be used in related studies of ACST.