红景天甙对体外培养的腺样囊性癌细胞的作用

Effect of salidroside on salivary adenoid cystic carcinoma cells in vitro

目的体外研究红景天甙对唾液腺腺样囊性癌细胞SACC-2生长的影响作用及其机制。方法采用CCK-8法测定红景天甙对体外培养的SACC-2细胞增殖的作用,并绘制细胞生长曲线;采用免疫组化方法检测红景天甙作用后SACC-2细胞中半胱氨酸蛋白酶(Caspase 3和Caspase 8)及增殖细胞核抗原(PCNA)蛋白表达情况。结果根据CCK-8测定法,红景天甙对SACC-2细胞增殖有抑制作用,IC50值为(4.99±0.23)μg/mL。生长曲线显示红景天甙组活细胞数随着培养天数增加而降低;红景天甙组SACC-2细胞的Caspase 3及Caspase 8蛋白表达均呈强阳性表达,对照组均为弱阳性表达,二组间差异显著(P<0.01);红景天甙组SACC-2细胞的PCNA阳性细胞数较对照组少,二组间差异显著(P<0.01)。结论红景天甙可能抑制了SACC-2细胞增殖及诱发其凋亡,有望成为临床抗癌药物之一。

Objective To study the effect and mechanism of salidroside on salivary adenoid cystic carcinoma （SACC-2） cells in vitro. Methods To detect the effect of salidroside on SACC-2 cells growth with CCK-8 kit, the growth curve of cells were drawn. To detect the expression of cysteine proteinase （Caspase 3 and Caspase 8） and proliferating cell nuclear antigen （PCNA） in SACC-2 cells, immunohistochemistry staining was used. Results According the results of CCK-8 kit detected, salidroside could inhibit the proliferation of SACC-2 cells, IC50 value was （4.99±0.23） μg/mL. Growing curve showed that SACC-2 cells of salidroside groups decreased with extending cell culture time. Immunohistochemistry staining showed that Caspase 3 and Caspase 8 were both strong positive expression in SACC-2 cells of salidroside groups, and poor positive expression in SACC-2 cells of control group, the difference was significant（P〈0.01）. The expression of PCNA was reverse（P〈0.01）. Conclusion Salidroside could inhibit the proliferation of SACC-2 cells and induce SACC-2 cell apoptosis in vitro, which could be a kind of antitumor medicine in the future.