牙周可疑致病菌对胶原包被羟磷灰石黏附的体外实验研究

Study of adherence of periodontal pathogens to collagen-treated hydroxyapatite

目的比较对牙根面具有黏附能力的具核梭杆菌、牙龈卟啉单胞菌、中间普氏菌和伴放线菌嗜血菌对胶原包被的羟磷灰石实验膜(C-HA)的黏附能力,初步探讨以上牙周可疑致病菌在牙根表面形成菌斑生物膜的能力。方法采用同位素闪烁计数法测定上述4种细菌黏附至C-HA表面的黏附量及黏附率,比较其黏附能力。结果培养相同时间,不论是培养24 h还是培养48 h,4种不同细菌两两比较,具核梭杆菌ATCC 10953和牙龈卟啉单胞菌ATCC 33277对C-HA表面黏附率的差异无统计学意义;中间普氏菌ATCC 25611和伴放线菌嗜血菌ATCC 29523的黏附率之间差异也无统计学意义,但是具核梭杆菌ATCC 10953和牙龈卟啉单胞菌ATCC 33277对C-HA表面的黏附率显著高于中间普氏菌ATCC 25611和伴放线菌嗜血菌ATCC 29523(P<0.001)。同一种细菌,在培养不同时间即培养24 h和48 h,对C-HA表面黏附率的差异均无统计学意义。结论不同的牙周可疑致病菌对胶原包被的羟磷灰石的选择性黏附作用不同,具核梭杆菌和牙龈卟啉单胞菌对胶原有较强的亲和作用,在细菌的局部定植过程和牙周炎的进展和复发中可能发挥重要作用。

Objective The ability of oral bacteria to adhere to tooth surface is associated with their pathogenicity. The objective of this study was to compare the ability of 4 strains of periodontal pathogens attaching to collagentreated hydroxyapatite （C-HA） beads in order to evaluate the ability of the main periodontal pathogens to form the biofilm on root surface. Methods The binding amount and binding percentage of 4 strains to C-HA were measured and compared by 3H-labeled binding assay. 4 strains of periodontal pathogens were Fusobacterium nucleatum （F nucleatum） ATCC 10953, Porphyrin gingivalis （P.gingivalis） ATCC 33277, Prevotella intermedia （P.intermedia） ATCC 25611 and Hemophilic.aetinomyceterncomitans （H.aetinomyceterncomitans） ATCC 29523.Results The differences of the percentage of relative adherence between F.nucleatum ATCC 10953 and P.gingivalis ATCC 33277, as well as between H.aetinomyceterncomitans ATCC 29523 and P.intermedia ATCC 25611 could not be observed. However, the percentage of relative adherence of F.nucleatum ATCC 10953 and P.gingivalis ATCC 33277 was higher than that of P.intermedia ATCC 25611 and H.aetinomyceterncomitans ATCC 29523 （P〈0.001）, no matter cultured 24 h or 48 h. No significant difference of the percentage of the relative adherence of each stain between 24 h and 48 h cultured time could be found. Conclusion F.nucleatum and P.gingivalis exhibited strong binding ability to C-HA. Their adherence to root surface may play an important role in their local aggregation, biofilm formation during the development and recurrence of the periodontitis.