巨噬细胞移动抑制因子在大肠黏膜癌变患者中表达增强

Increased expression of macrophage migration Inhibitory factor in epithelial and serum in patients with colorectal carcinoma

目的探讨巨噬细胞移动抑制因子(MIF)表达与大肠癌临床病理因素的关系。方法采用免疫组化法测定大肠组织中MIF的表达。ELISA测定血清中MIF水平。结果MIF在正常大肠黏膜、大肠腺瘤、大肠癌中阳性表达强度和阳性表达率依次增高,3组之间存在显著性差异((P<0.001);组织中MIF表达强度与血清中MIF水平呈正相关性;MIF的表达与大肠癌的分化程度、淋巴结转移、肝转移有关。结论MIF可能在大肠癌的发生和进展中起着重要的作用。

Objective To determine MIF expression in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma, and the relationship between MIF and the clinical pathologic features of colorectal carcinoma. Methods MIF expression was determined by immunohistochemical staining ELISA was used to detect the level of MIF in serum samples. Results The percentage of MIF expressing epithelial cells and MIF positive expression intensity were progressively increased in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma. There was significant difference among three groups （P 〈 0. 001, ANOVA）. There was a significant association between epithelial MIF expression and serum MIF level（ R =0. 751, P =0. 002）. The expression of MIF was positively correlated with differentiation degree, lymph node metastasis, and liver metastasis. Conclusion MIF is involved in colorectal carcinogenesis and development.