摘要
目的:检测慢性心力衰竭患者(CHF)外周血白细胞介素(IL)-12和IL-18的水平并探讨其意义。方法:CHF患者72例,按照NYHA心功能分级,分为HF1组(NYHA Ⅱ~Ⅲ级),HF2组(NYHA Ⅳ级)。按照病因分类,分为缺血性心脏病(IHD)组(43例)和非缺血性心脏病(NIHD)组(29例)。选取29例健康志愿者作为正常对照组(C组)。采用双抗体夹心酶联免疫吸附法(ELISA)检测血清中IL-12和IL-18的水平,用彩色多普勒超声诊断仪测定左室舒张末期内径(LVEDd)和左室射血分数(LVEF)。结果:HF1、HF2、IHD和NIHD组IL-12的水平显著升高,分别为(34.56±19.10),(35.12±15.18),(31.18±7.80),(32.73±11.54)ng/L,与C组(28.73±13.43)ng/L比较,均差异有统计学意义(P〈0.05),IL-18的水平分别为(39.50±5.66),(46.76±12、66),(40、58±13.10),(42.53±6.16)ng/L,明显高于C组(31.82±5.79)ng/L,均差异有统计学意义(P〈0.05或P〈0.01),HF2组血清IL-18水平显著高于HF1组(P〈0.05)。与C组比较,HF1、HF2、IHD和NIHD组的LVEDd明显增大,LVEF显著降低。IL-12水平与LVEDd呈低度正相关(P〈0.01),IL-18水平与LVEDd呈中度正相关(P〈0.01),与LVEF呈中度负相关。结论:IL-12和IL-18在CHF患者血清中表达升高;IL-12的水平与患者基础病因和心功能均无明显相关性;IL-18的水平与CHF的基础病因无明显相关性,但与患者的CHF程度呈正相关。
Objective:To investigate the serum levels of IL-12, IL-18 in patients with chronic heart failure, and explore the roles of the cytokines in the pathogenesis of chronic heart failure. Method: Seventy-two patients with chronic heart failure were divided into 2 groups according to the New York Heart Association (NYHA) classification system: group HF1 ( Ⅱ-Ⅲ ) and group HF2 (Ⅳ), of which 43 patients with ischemic heart disease (group IHD), 29 with non-ischemic heart disease (group NIHD). Twenty-nine healthy donors were enrolled as group C. The serum levels of IL-12 and IL-18 in the patients and donors were detected by ELISA. Result: The results showed that:①The level of IL-12 in group IHD and NIHD is (31. 18±7.80)ng/L,(32.73± 11.54)ng/L; were significantly higher than group C (28.73±13.43)ng/L (P〈0.05), so were in group HF1 and HF2 (HF1[34.56 ±19.10]ng/L; HF2 [35.12 ± 15.18] ng/L) compared with group C (P 〈 0.05). But there was no significant difference between group HF1 and HF2, so were in group IHD and NIHD; ②The level of IL-18 is significantly higher in group IHD (40, 58±13, 10)ng/L and group NIHD (42.53±6.16)ng/L compared with group C (31.82 ±5.79)ng/L( P〈0.05), but there was no obvious difference between group IHD and NIHD while there was significant difference between group HF1 and HF2. Conclusion:The serum levels of IL-12 and IL-18 were elevated in chronic heart failure patients. The level of IL-18 was related with the heart function. Both IL-12 and IL-18 might participate in the development of heart failure.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2008年第7期494-496,共3页
Journal of Clinical Cardiology
基金
国家自然科学基金资助(No:30600234)
国家重点基础研究发展计划(973计划)资助(No:2007CB512000
2007CB512005)