Livin蛋白在原发性肝细胞癌组织中的表达和意义

Expression of livin in hepatocellular carcinoma and its significance

目的探讨凋亡抑制蛋白（inhibitor of apoptosis protein，IAP）Livin在人原发性肝癌（HCC）组织中的表达及其与HCC血管生成的关系。方法应用免疫组织化学法检测42例HCC及对应癌旁组织和12例正常肝组织内Livin的表达情况，观察Livin蛋白与HCC病理学特点及微血管密度（mierovessel density，MVD）之间的关系。结果免疫组织化学（SP）染色结果显示，Livin蛋白在正常肝组织中均不表达，而在42例HCC中的阳性表达率（73．81％）显著高于癌旁组织（4．76％）和正常肝脏组织，有显著统计学意义（x^2=39．13，P〈0．05；x^2=17．89，P〈0．05）；Livin蛋白在病理分级Ⅲ～Ⅳ组阳性表达率（85．12％）强度明显高于病理分级Ⅰ～Ⅱ组（53．33％），两者比较差异有显著统计学意义（x^2=4．690，P〈0．05）；Livin蛋白在有转移HCC中的阳性表达率（89．47％）明显高于无转移组（60．89％），有统计学意义（x^2=4．404，P〈0．05）；Livin蛋白阳性表达HCC中的MVD值明显高于Livin蛋白阴性表达HCC中MVD值（23．56±5．12／17．63±4．86；P〈0．05）。结论Livin蛋白基因在原发性肝癌组织中高表达，提示该基因可能参与了肝癌的发生发展过程；Livin蛋白在HCC组织中的表达明显上调且与血管生成和浸润转移有关。

Objective To explore the expression of livin in hepatocellular carcinoma（HCC）. Methods With the method of immunohistochemical staining, specimens from 42 HCC patients and their corresponding para-carcinoma liver tissues and 12 normal liver controls were studied. The relationship between the livin expression and pathological features as well as microvessel density（MVD） was evaluated. Results The expression of livin in the HCC tissues was much higher than that in the corresponding para-carcinoma liver tissues（x^2 = 39.13, P〈0.05）and normal liver tissues（x^2 = 17.89, P〈0.05）. Over expression of livin in HCC was related to the invasion of tumor Edmondson grades （X^2 = 4. 690, P〈0.05） and tumor metastasis （X^2 = 4. 404, P〈 0.05）. MVD of HCC with positive expression of llvin was much higher than that in HCC with negative expression of livin（P〈0.05）. Conclusion The up-regulated expression of the livin protein gene in HCC suggests that livin participates in carcinogenesis. Livin may play a crucial role in the angiogenesis, invasion and metastasis of HCC.