头穴丛刺法对急性脑梗死大鼠病理形态学及凋亡相关基因Caspase-3的影响

Effects of Scalp Cluster needling on Pathomorphology and Apoptosis-related Gene Caspase-3 in Rats with Acute Cerebral Infarction

目的通过观察头穴丛刺对急性脑梗死大鼠病理形态学及凋亡相关基因Caspase-3表达的影响,探讨该方法治疗脑梗死的作用机制。方法将72只雄性Wister大鼠随机分为三组:假手术组(A)、模型组(B)、头穴丛刺组(C),每组24只。各组再按脑梗死后不同时间点分为6 h、24 h、3 d三个亚组,每组8只。采用线栓法制备急性脑梗死动物模型,观察头穴丛刺法对急性脑梗死大鼠不同时间点缺血侧脑组织病理形态学及凋亡相关基因Caspase-3表达的变化。结果脑梗死第3 d,B组大鼠梗死灶中心区坏死彻底,周围区神经细胞数目明显减少,脑组织水肿明显,可见神经毡疏松、淡染,与B组比较,C组大鼠梗死灶周边可见胶质细胞增生,脑组织水肿减轻;脑梗死后24 h至3 d,C组大鼠缺血侧皮层促凋亡基因Caspase-3表达明显减少,与B组比较,差异均有统计学意义(P<0.01)。结论头穴丛刺疗法能够改善脑梗死缺血半暗区神经细胞功能状态,促进神经胶质细胞的增生;并可通过抑制促凋亡基因Caspase-3的表达,而发挥一定的脑保护作用。

Objective To investigate the mechanism of action of scalp cluster needling in the treatment of cerebral infarction by observing its effects on pathomorphology and apoptosis-related gene caspase-3 in rats with acute cerebral infarction, Methods Seventytwo female rats were randomly allocated to a sham-operation group （ A）, a model group （B） and a scalp cluster needling group （ C）, 24 rats each. According to different times after cerebral infarction, each group was reassigned to 3 subgroups： 6 h, 24 h and 3 d, 8 rats in each subgroup. A animal model of acute cerebral infarction was made by a thread-occlusion method, The effects of scalp cluster needling on pathomorphology and expression of apoptosis-related gene caspase-3 in the brain tissue on the ischemic side at different times were investigated in the rats with acute cerebral infarction. Results In group B rats, complete necrosis appeared in the central zone of infarction, and the number of neurons decreased significantly and the brain tissue showed obvious edema and loose and lightstained neuropil in the peripheral zone, at 3 days after cerebral infarction. Compared with group B, glial cells proliferated and brain tissue edema abated in the peripheral zone of infarction in group C rats, At 24h to 3d after cerebral infarction, the expression of apoptosis-promoted gene caspase-3 reduced obviously in the cortex on the ischemic side in group C rats, There was a statistically significant difference compared with group B （P 〈 0.01 ） ： Conclusion Scalp cluster needling can improve neuronal function and promote neuroglial cell proliferation in the penumbra zone of cerebral infarction, It plays a certain role in cerebral protection by inhibiting the expression of apoptosis-promoted gene caspase-3.