芒果成熟阶段蔗糖代谢及其相关酶类研究

Sucrose metabolism in Irwin Mango(Mangifera indica L.) during maturation

以爱文芒果(Irwin)为试材,研究了爱文芒成熟阶段的蔗糖代谢。结果表明:爱文芒果实中可溶性糖含量最多的是蔗糖(81.79 mg.g-1)、果糖次之(30.15 mg.g-1),葡萄糖最少(9.95 mg.g-1)。爱文芒的酸性转化酶(AI)、中性转化酶(NI)、蔗糖合成酶(SS)、蔗糖磷酸合成酶(SPS)在采收前活性差别不大,在后熟阶段,AI、SS、SPS活性显著增加,并且SPS活性达到最高值,NI略有增加。葡萄糖含量采后呈先增后减的趋势,果糖含量采前缓慢增加,采后迅速增加,蔗糖含量在采前略有增加,采后呈快速积累趋势,并且含量达到最高值。爱文芒果的糖代谢关键时期是采后阶段,并且以积累蔗糖为主,SPS起关键作用。

Sucrose metabolism of Irwin mango （Mangifera indica L. ） during maturation was studied. The results showed that the content of sucrose was the highest （81.79 mg/g）, the next was fructose （30.15 mg/g）, and the lowest was glucose （9.95 mg/g） in the soluble sugar of mango. Although the activites of acid invertase （ AI）, neutral invertase （ NI）, sucrose synthase （ SS）, sucrose phosphate synthase （SPS） were no difference before the harvest of mango, the activities of AI, SS, SPS increased significantly and NI increased slightly during the postharvest period, especially SPS activity got to the maximum. The glucose increased and later decreased during the postharvest period, fructose increased slightly before harvest and sharply at postharvest, which was the same as the changes of sucrose, and sucrose had a maximum at harvest. Sugar of mango metabolized at the postharvest period mainly, and sucrose of which were accumulated mainly, SPS was a key to contribute significantly to sucrose metabolism.