猪繁殖与呼吸障碍综合征病毒美洲株与猪Ⅱ型圆环病毒混合感染仔猪的血液病理学研究

Study on hematopathology of piglets infected with porcine reproductive and respiratory syndrome virus(ATCC VR-2332) and porcine circovirus type 2

选取3周龄左右早期断奶仔猪单独感染猪繁殖与呼吸障碍综合征病毒（PRRSVATCCVR-2332株）并与猪Ⅱ型圆环病毒（PCV2）人工共同按种，在不同时期取外周血，进行外周血常规生理指标和血液生化指标的检测，比较不同按种组中各指标的差异性。外周血淋巴细胞采用碘化丙啶（PI）单染色法染色，借助流式细胞仪检测细胞凋亡情况。结果表明，两病毒感染对外周血各种指标都有一定的影响，WBC、GPT、GOT、LDH、GLO及TBIL感染组高于对照组；RBC、HGB、PLT及ALB感染组低于对照组；PRRSV单独感染以及与PCV2在混合感染的变化规律基本相同，但混合感染多项指标的变化幅度更大；MCV及TP无明显差异；两病毒接种组外周血淋巴细胞的凋亡比率显著高于对照组，攻毒后2～3d达到高峰，此后逐渐下降并趋于恢复，混合感染组的凋亡比率又高于单独感染组。文章就这些病理现象的发生及影响进行了分析讨论。

Three-week-old weaned piglets were inoculated with porcine reproductive and respiratory syndrome virus （PRRSV ATCC VR- 2332） independently and combined with porcine cireovirus type 2 （PCV2） ,peripheral blood of different periods were adopted and physico- chemical properties were detected and compared with each other,respectively. PI single staining technique was used to detect the quantity of apoptotic lymphocytes. The detecting results of physico-chemieal properties showed that virus inoculation influenced the most of the physicochemical properties of peripheral blood,the effects of WBC, GPT, GOT, LDH, Gig） and TBIL were higher than that of the control,the effects of RBC, HGB,PLT and ALB were lower than that of the contrul,the change tenderteies of virus inoculating groups were almost the same and the changes of mixed inoculating group were greater than that of the PRRSV inoculating group. The effects of MCV and TP were normal. Apoptosis ratio of virus inoculating group could reach to the highest level in about 2 -3 days and then declined to the normal level. The ratio of apoptotie lymphocytes from mixed inoculation group was higher than that of single inoculation group ,the genesis and effect of pathologic phenomena were also discussed in the article.