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腺病毒E1区对病毒生产能力影响的初步研究

A Pilot Study to Determine the Effect of Adenoviral E1 Region on Adenovirus Productivity
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摘要 腺病毒载体具有高效转染和低细胞毒性的特点,已成为基因治疗研究中应用最广泛的载体之一。作为最新一代腺病毒载体,第三代腺病毒载体由于其生产效率低下,难以生产临床使用的治疗剂量,所以一直没有临床实验的结果报道。为了提高腺病毒的生产效率,文章研究E1区对腺病毒在CA293细胞中生产能力的影响,结果发现E1区基因拷贝数和蛋白表达与腺病毒的产量呈正相关,而不影响病毒DNA在CA293中的复制能力,推测E1区影响病毒DNA复制后的装配和释放过程,为改进腺病毒的生产方法提供理论基础。 Adenovirus vectors have the characteristics of high transfection efficiency and low cytotoxicity, and they have become most widely used vectors for gene therapy. As the last generation adenovirus vector, the third generation can hardly increase to therapeutic dose for its low productivity, and thus no clinical report of it has been published by far. In order to improve adenovirus productivity, the authors study the effect of E1 region on adenoviral production capacity in CA293 cell line. It is observed that E1 region gene copies and protein expression are positively related to adenoviral production while they have no influence on viral DNA replication in CA293, indicating that E1 region affects the process of virus assembly and release. The research provides the theoretical basis for advances in procedures of adenoviral production.
作者 尹跃翔 钱程
机构地区 浙江理工大学生命科学院
出处 《浙江理工大学学报（自然科学版）》 2008年第5期554-558,共5页 Journal of Zhejiang Sci-Tech University(Natural Sciences)
关键词 腺病毒栽体 E1区 腺病毒生产效率 病毒DNA复制 adenovirus vector E1 region adenoviral productivity viral DNA replication
分类号 Q782 [生物学—分子生物学]
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参考文献7

  • 1[1]Edelstein M L,Abedi M R,Wixon J,et al.Gene therapy clinical trials worldwide 1989-2004-an overview[J].The Journal of Gene Medicine,2004,6(8):597-602.
  • 2[2]Hardy S,Kitamura M,Harris-Stansil T,et al.Construction of adenovirus vectors through Cre-lox recombination[J].Journal of Virology,1997,71(3):1842-1849.
  • 3[3]He T C,Zhou S,da Costa L T,et al.A simplified system for generating recombinant adenoviruses[J].Proceedings of the National Academy of Sciences of the United States of America,1998,95(5):2509-2514.
  • 4[4]Danthinne X,Imperiale M J.Production of first generation adenovirus vectors:a review[J].Gene Therapy,2000,7(20):1707-1714.
  • 5[5]Cui Q,Jiang W,Wang Y,et al.Transfer of suppressor of cytokine signaling 3 by an oncolytic adenovirus induces potential antitumor activities in hepatocellular careinoma[J].Hepatology,2008,47(1):105-112.
  • 6刘金,刘必胜,刘新垣.IRES连接双基因在溶瘤腺病毒中的抗肝癌作用[J].浙江理工大学学报（自然科学版）,2007,24(4):466-470. 被引量：3
  • 7[7]Puntel M,Curtin J F,Zirger J M,et al.Quantification of high-capacity helper-dependent adenoviral vector genomes in vitro and in vivo,using quantitative TaqMan real-time polymerase chain reactior[J].Human Gene Therapy,2006,17(5):531-544.

二级参考文献17

  • 1[1]Ngoi S M,Chien A C,Lee C G L.Exploiting internal ribosome entry sites in gene therepy vector design[J].Curr Gen Ther,2004(4):15-31.
  • 2[2]Emerman M,Temin H M.Genes with promotors in retrovirus vectors can be independently supressed by an epigenetic mechanism[J].Cell,1984,39:449-67.
  • 3[3]Morgan R A,Couture L,Elroy-Stein,et al.Retroviral vectors containing putative internal ribosime entry sites:development of a polycistronic gene transfer system and applications to huamn gene therapy[J].Nucleic Acids Res,1992,20:1293-1299.
  • 4[4]Jiang H,Lin J J,Su Z Z,et al.Subtraction hybridization identifies a novel melanoma differentiation associated gene,mda-7,modulated during human melanoma differentiation,growth and progression[J].Oncogene,1995,11:2477-2486.
  • 5[5]Su Z Z,Lebedeva I V,Gopalkrishnan R V,et al.A combinatiorial approach for selectively inducing programmed cell death in human pancreatic cancer cells[J].Proc.Natl.Acad.Sci.U.S.A.2001,98:10332-10337.
  • 6[6]Su Z Z,Madireddi M T,Lin J J,et al.The cancer growth suppressor gene mda-7 selectively induces apoptosis in human greast cancer cells and inhibits tumor grwth in nude mice[J].Proc.Natl.Acad.Sci.U.S.A.1998,95:14400-14405.
  • 7[7]Madireddi M T,Su Z Z,Yong C S,et al.A novel melanoma differentiation associated gene with promise for cancer gene therapy[J].Adv Exp Med Biol,2000,465:239-261.
  • 8[8]Saeki T,Mhashilkar A,Swanson X,et al.Inhibition of human lung cancer growth following adenovirus-mediated mda-7 gene expression in vivo[J].Oncogene 2002,21:4558-4566.
  • 9[9]Leath C A,Kataram M,Bhagavatula P,et al.Infectivity enhanced adenoviral-mediated mda-7/IL-24 gene therapy for ovarian carcinoma[J].Gynecol Oncol,2004,94:352-362.
  • 10[10]Su Z Z,Lebedeva I V,Sarkar D,et al.Melanoma differentiation associated gene,mda-7/IL-24,selectively induces growth suppression,apoptosis and radiosensituzation in malignant gliomas in a p53-independent manner[J].Oncogene,2003,22:1164-1180.

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浙江理工大学学报（自然科学版）
2008年 第5期

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