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唾液富组蛋白5表达载体的构建

Construction of Expression Vector of Salivary Histatin 5
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摘要 目的将唾液富组蛋白5 cDNA克隆至表达载体pGEX-4T-1。方法EcoRⅠ+SalⅠ双酶切克隆载体pMD19-T-hrp5及pGEX-4T-1,回收目的片段hrp5及双酶切载体pGEX-4T-1,将二者连接后转化大肠杆菌DH5α,氨苄青霉素筛选阳性菌落,PCR、酶切、测序鉴定重组质粒。结果唾液富组蛋白5的cDNA正确克隆至表达载体pGEX-4T-1,无突变。结论重组载体pGEX-4T-1-hrp5构建成功。 Objective To clone the eDNA of salivary histatin 5 to expression vector pGEX-4T-1. Meth- ods pMD19-T-hrp5 and pGEX-4T-1 were digested by Sal I + EcoR. The digested hrp5 and pGEX-4T-1 were linked and transformed to E. coli DH5α ,and the positive colonies were screened by ampiciUin, and identified by PCR, digestion and sequencing. Results The eDNA of hitatin 5 was correctly inserted into expression vector pGEX-4T-1 without mutation. Conclusion Recombinant vector pGEX-4T-1-hrp5 was constructed successfully.
出处 《咸宁学院学报(医学版)》 2008年第3期191-193,共3页 Journal of Xianning Univarsity(medical Sciences)
关键词 唾液富组蛋白5 克隆 表达载体 Salivavry Histatin 5 Clone Expression vector
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参考文献2

  • 1Oppenheim FG, Xu T, McMillian FM, et al. Histatins, a novel family of histidine-rich proteins in human parotid secretion, isolation, characterization, primary structure and fungistatic effects on Candida albicans [ J ]. Biol Chem, 1988,263:7472
  • 2金科华,周琦,罗德生,王彪,程新潮,段海瑞.唾液富组蛋白5克隆载体的构建[J].咸宁学院学报(医学版),2007,21(5):385-387. 被引量:1

二级参考文献2

  • 1赵亚华,董竟南,崔红,白云,金科华,蒋智勇.蜂毒素分子的改造及其基因在毕赤酵母中的表达[J].中国生物工程杂志,2005,25(2):45-48. 被引量:8
  • 2Oppenheim FC,Xu T,McMillian FM et al.Histatins,a novel family of histidine-rich proteins in human parotid secretion,isolation,characterization,primary structure and fungistatic effects on Candida albicans[J].Biol Chem,1988,263:7472

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