手术操作与胃癌微转移的关系

SURGICAL MANEUVERS AND MICROMETASTASIS OF GASTRIC CANCER

目的探讨手术操作与胃癌微转移的关系。方法采用巢式逆转录-聚合酶链式反应(RT-PCR)技术检测70例胃癌病人手术前、后外周血细胞角蛋白(CK20)mRNA及癌胚抗原(CEA)mRNA的表达情况,并以20例健康志愿者作为阴性对照组,以10例胃癌组织作为阳性对照组。结果CK20 mRNA及CEA mRNA在术前阳性表达率分别为32.9%和51.4%,而术后二者的阳性表达率分别为62.9%和70.0%,术后阳性率明显高于术前(χ2=12.62、5.06,P<0.05)。20例健康志愿者无1例阳性表达。结论手术操作可以促使胃癌发生微转移。

Objective To study the relationship between surgical maneuvers and micrometastasis of gastric cancer, Methods Using nested reverse transcription polymerase chain reaction, the expressions of CK20 mRNA and CEA mRNA in peripheral blood were determined in 70 gastric cancer patients, before and after operation. Twenty healthy volunteers served as positive controls. Results Of 70 patients operated, the expressions of CK20 mRNA and CEA mRNA were 32.9% and 51.4%, respectely preoperatively; while postoperatively, they were 62.9 % and 70.0 % （X^2 = 12.62,5.06 ; P〈0.05）. No positive expressions were noted in healthy controls. Conclusion Surgical maneuvers may cause micrometastasis in gastric cancer.