RP-HPLC法测定冰蟾乳膏中马钱子碱和士的宁的含量

Determination of Brucine and Strychnine in BingChan Cream by RP-HPLC

目的：建立冰蟾乳膏中马钱子碱和士的宁的含量测定方法。方法：采用DiamonsilTMC18色谱柱（250 mm×4.6 mm,5μm）,流动相为乙腈-0.015 mol/L庚烷磺酸钠与0.02 mol/L磷酸二氢钾等量混合溶液（含0.1%三乙胺,用10%磷酸调节pH值至3.0）（20∶80）,流速：1.0 mL/min,检测波长：256 nm。结果：马钱子碱在2.4-24 mg/L范围内（r=0.999 7）、士的宁在2.95-29.5 mg/L范围内（r=0.999 9）线性关系良好,其平均加样回收率分别为98.3%和97.9%,RSD分别为1.54%和2.20%（n=5）。结论：本法快速、准确、重复性好,可作为冰蟾乳膏的质量控制方法。

Objective： To establish a method for the determination of brucine and strychnine in BingChan cream by RP-HPLC. Methods： A DiamonsilTM C18 column（250 mm ×4.6 mm, 5 um） was used, the mobile phase was acetonitrile-equivalent mixture consisted of 0.02 mol/L KH2PO4 and 0. 015 mol/L sodium heptanesulfonate with 0.1% triethylamine （pH 3.0） （20： 80）, the flow rate was 1.0 mL/ min, and the detection wavelength was 256 nm. Results： The linear range of brucine and strychnine was 2.4-24 mg/L （r =0.9997） and 2.95-29.5 mg/L （r = 0. 999 9）, respectively, and the mean recovery was 98.3% （ RSD = 1.54%） and 97.9% （ RSD = 2.20%）, respectively. Conclusion： The method is simple, accurate and reliable for the quality control of BingChan cream.