灯盏花素对脑缺血再灌注沙土鼠相关行为指标影响

EFFECT OF BREVISCAPINE ON THE ETHOLOGICAL INDEX AFTER CEREBRAL ISCHEMIA/REPERFUSION IN GERBILS

目的研究灯盏花素注射液对沙土鼠脑缺血再灌注后相关行为影响。方法制备沙土鼠前脑缺血再灌注模型,脑缺血时间为10 min。将沙土鼠随机分为假手术组、对照组和灯盏花素组,每组8只动物,后两组再根据再灌注时间分为再灌注1、2、4、7 d等4个亚组。用开阔法观察沙土鼠脑缺血再灌注后行为学改变。实验期间始终将沙土鼠脑温控制在(37.2±0.2)℃。结果沙土鼠脑缺血再灌注后1、2、4和7 d,对照组相关行为学指标评分明显高于假手术组,而灯盏花素组沙土鼠行为学评分尽管高于假手术组,但明显低于对照组。结论灯盏花素可减轻脑缺血再灌注对沙土鼠相关行为学指标的影响,减轻沙土鼠脑缺血再灌注损伤。

Objective To investigate the effect of breviscapine on the ethological index after cerebral ischemia/reperfu sion in gerbils. Methods A cerebral ischemia reperfusion model of gerbils was established, ischemia time being 10 minutes. Gerbils were randomised to sham group, control group and breviscapine group. They were further divided into four subgroups, eight in each group. Reperfusion was carried out in the latter two groups, which lasted for 1, 2, 4, and 7 d, respectively. The ethological index of gerbils was counted by the open field method after reperfusion. The brain temperature in all gerbils was kept at（ 37.2± 0.2） ℃ during the experiment. Results After different time of reperfusion, the score of ethological index in control group was higher than that in sham group, that in breviscapine group was higher than sham group, but was lower than control group. Conclusion Breviscapine could reduce cerebral ischemia＇reperfusion injury in gerbils by attenuating the changes of the behavioral marks.