肉苁蓉HPLC指纹图谱研究

Study on HPLC fingerprint of Cistanche deserticola

目的:建立肉苁蓉的HPLC指纹图谱分析方法。方法:以毛蕊花糖苷为参照物,采用梯度洗脱HPLC法。色谱条件如下:Shim-pack VP-ODS色谱柱(150 mm×4.6 mm,5μm);流动相A为甲醇-乙腈(50∶50),B为0.05 mol/L乙酸钠缓冲液(冰乙酸调pH值至4.0),梯度洗脱;检测波长:334 nm。结果:方法的精密度、稳定性、重现性较好,共标示出肉苁蓉27个共有峰。结论:该方法可用于肉苁蓉药材的质量控制。

Objective：To establish a method of HPLC fingerprint analysis of Cistanche deserticola. Methods： With acteoside as the reference substance, gradient elution HPLC method was applied to establish fingerprint chromatography of Cistanche deserticola. Shim-pack VP-ODS column（150 mm × 4. 6 mm,5 μm）was used. Mobile phase A （methanol ： acetonitrile=50 ： 50） and mobile phase B,0.05 mol/L sodium acetate buffer, whose pH value was adjusted to 4.0 with glacial acetic acid,were adopted as the mobile phase. Detection wavelength was 334 nm. Results： The method had good precision, stability and repeatability. Twenty-seven common peaks were found in the HPLC fingerprint of Cistanche deserticola. Conclusion：The method can be used for the quality control of Cistanche deserticola.