复方二氧化钛软膏中防晒剂的鉴别和含量测定

Identification and quantitative determination of sunscreen agents in compound titanium dioxide ointment

目的：建立复方二氧化钛软膏中防晒剂的鉴别和含量测定方法。方法：以显色反应鉴定二氧化钛，采用HPLC法同时测定二苯酮-3（MC40）和甲氧基肉桂酸辛酯（MC80）的含量。色谱柱为Diamonsil C18柱（200min×4．6mm，5μm）；流动相为甲醇：水（90：10）；流速1．0mL／min；柱温35℃；检测波长0～9min为287nm，9～20min为309nm。结果：MC40和MC80分别在30-300μg／mL和100～1000 μg／mL范围内呈良好的线性关系。精密度、回收率、重复性和稳定性均符合方法学要求。结论：该方法准确、可靠、快速，适用于复方二氧化钛软膏的质量控制。

Objective：To establish a method for identification and quantitative determination of sunscreen agents in com pound titanium dioxide ointment. Methods： Titanium dioxide was identified by color reaction. HPLC method was used for simultaneous determination of benzophenone-3 （MC40） and octyl methoxycinnamate （ MC80 ）. A Diamonsil C18 column （200 mm × 4.6 mm,5 μm） was used. The mobile phase was methanol-water（90 ： 10） at the flow rate of 1.0 mL/min. The gradient detec tion wavelengths were at 287 nm in 0 9 min and 309 nm in 9-20 min. Results：Good linearities were obtained in the ranges of 30- 300 μg/mL and 100-1 000 μg/mL for MC40 and MC80, respectively. The precision, recovery, reproducibility and stability met the requirements of methodology. Conclusion： This method is accurate, reliable, rapid and suitable for the quality control of compound titanium dioxide ointment.