阿奇霉素与环丙沙星合用对金黄色葡萄球菌生物膜的影响

Synergism of Azithromycin and Ciprofloxacin on staphylococcus aureus biofilm

目的研究阿奇霉素对金黄色葡萄球菌生物膜的影响及与环丙沙星合用后的抗菌协同作用。方法用0.22μm微孔滤膜构建金黄色葡萄球菌生物膜模型;测定阿奇霉素、环丙沙星的最低抑菌浓度(MIC)值;银染金黄色葡萄球菌生物膜,扫描电镜(SEM)观察生物膜形态结构,连续稀释法进行活菌计数。结果生物膜(BF)可在七天内稳定形成,1/16MIC、1/4MIC浓度阿奇霉素显著减少单用环丙沙星组中的BF活菌数(P<0.05)。结论阿奇霉素能显著提高环丙沙星对BF中金黄色葡萄球菌的抗菌活性。

Objective To investigate the influence of azithromycin on staphylococcus aureus bio-film formation and synergism of antibacterial activites between azithromycin and ciprofloxacin on staphylococcus aureus. Methods Staphylococcus aureus biofilm were cultivated with 0.22μm aperture micropore film in vitro and measured the MICs of azithromycin and ciprofloxacin; staphylococcus aureus BF was stained with AgNO3 and the morphological structure was observed by Scanning electron microscopy（SEM）;The viable bacterial in BF were counted separately by serial dilution. Result The BF of staphylococcus aureus formed steadily after a week. The bactericidal activity of ciprofloxacin to staphylococcus aureus within the BF was enhanced significantly when azithromycin of 1/16M IC and 1/4 MIC combined with ciprofloxacin alone was applied respectively（P〈0.05）. Conclusion Azithromycin was considered to enhance significantly the bacterial sensitivity to ciprofloxacin on staphylococcus aureus in biofilm.