丹参川芎嗪对胰腺炎患者血清IL-1β和TNF-α含量的影响

Serum IL-1β and TNF-α levels and their changes in the treatment of AP patients with Salivae Miltiorrhizae Liguspyragine

目的:通过检测急性胰腺炎(acute pancreatitis,AP)患者外周血清IL-1β和TNF-α的含量的变化,探讨二者与AP的关系及中药制剂对二者变化的影响。方法:选择AP患者40例,随机分为一般治疗组和参芎组,另选健康体检者做对照组,分别于入院后第1天及第7天抽取外周静脉血2mL,采用双抗体夹心EL ISA法检测血清中IL-1β和TNF-α的含量,同时记录AP患者一般状况及生化指标。结果:入院第1天AP组IL-1β和TNF-α的含量明显增高,与正常对照组比较有显著性差异(P<0.01);治疗7d后,IL-1β和TNF-α含量均下降,参芎组较一般治疗组下降明显(P<0.05)。结论:急性胰腺炎患者血清中IL-1β和TNF-α的含量均增高,应用参芎葡萄糖注射液结合常规治疗可明显降低IL-1β、TNF-α水平,减轻胰腺损伤。

Objective：To investigate the relationship between acute pancreatitis（AP） and the serum IL-1β and TNF-α levels and their changes of these cytokines in the treatment with Salivae Miltiorrhizae Liguspyragine Hydrochloride and Glucose injection,and study the role of Chinese medicine in the changes of in the serum IL-1βand TNF-αlevels in patients with AP. Methods：Forty patients with AP were randomly divided into three groups： the general treatment group, the treatment group and the control group （10 healthy subjects）. 2 ml peripheral vein blood from all the patients was collected at 1 and 7 days after their admission. The serum IL-1βand TNF-αlevels were detected by using double antibody sandwich ELISA and at the same time,their general condition and biochemical indicators were recorded. Resul ts：At the first day after admission, the serum IL-1β and TNF-αlevels in AP group were increased ,compared with control group, with a significant difference （P 〈 0.01） . After seven--day treatment, the IL-1β and TNF-αlevels in the treatment group （Salivae Miltiorrhizae Liguspyragine Hydrochloride group） were decreased,compared with the general group （P 〈 0.05） . Conclusion：The serum IL-1βand TNF-αare involved in the development of AP process,and their levels are higher in AP patient serum,the application of Senate Ligusticum glucose injection combined with conventional therapy can significantly reduce IL-1β and TNF-α levels,lessening pancreas injury.