垂体腺苷酸环化酶激活肽对MCAO大鼠自体神经干细胞分化影响

Effects of Pituitary adenylate cyclase-activating polypeptide on the differentiation expression of autoallergic neural stem cells in MCAO rat

目的:研究垂体腺苷酸环化酶激活肽(PACAP)对MCAO大鼠自体神经干细胞分化影响。方法:将大鼠随机分为不同浓度PACAP治疗组:P8组(1×10-8mol/L),P10组(1×10-10mol/L),P12组(1×10-12mol/L);对照组。免疫荧光双标观察BrdU+/NSE+及BrdU+/GFAP+细胞的表达变化。结果:各组BrdU+/NSE+及BrdU+/GFAP+细胞7d时较少,14d显著增加,28d时最高,PACAP治疗组高于对照组(P<0.01);P8组高于P10组,P12组(P<0.05)。结论:PACAP具有诱导神经干细胞分化的作用,以1×10-8mol/L为最佳浓度。

Objective： To investigate the effect of Pituitary adenylate cyclase- activating polypeptide （PACAP） on the differentiation expression of neural stem cells in MCAO rat. Methods：Rats were randomly divided into different concentrations of PACAP treatment groups： 8group （1 × 10^-8mol/L）, P10group （1 × 10^-10mol/L） ,and P12 group （1 × 10^12mol/L） and control group. Imunofluorescence staining was applied to detection of the differential espression of Brdu^＋/NSE^＋ and Brdu^＋/GFAP^＋ positive cells. Results：At 7d, there were fewer BrdU^＋/NSE^＋ and BrdU^＋/GFAP^＋ positive cells in all groups,slgnlficantly increasing at 14d,and reaching the peak at 28d. The PACAP treatment groups were higher than the control group （P 〈 0.01） ;P8 group was higher than P10 group and P12 group （P 〈 0. 05） . Conelusion：PACAP has an obvious effect in inducing differentiation of neural stem cells, and PACAP shows the optimal effect at its concentration of 1 × 10^-8 mol/L.