紫杉醇对血管平滑肌细胞原癌基因转录和表达的影响

The Effect of Paclitaxel on the Transcription and Expression of VSMC Protooncogene

目的观察紫杉醇（paclitaxel）对血管平滑肌细胞原癌基因c-jun、c-fos转录和表达的影响及其量效、时效关系。方法提取大鼠主动脉原代平滑肌细胞，将第4-9代细胞用于实验。分别运用逆转录聚合酶链反应和免疫组织化学技术检测血管紧张素及不同浓度（O．1、1．0和10．0tanol／L）紫杉醇对平滑肌细胞c-iun、c-fos mRNA转录及其蛋白质产物合成的影响。结果高、中、低剂量紫杉醇对血管紧张素诱导原癌基因的转录和表达没有明显作用。结论血管紧张素通过促进c-fos、c-jun的转录和表达引起血管平滑肌细胞的增殖。紫杉醇抗血管平滑肌细胞增殖作用并非通过抑制原癌基因c-fos、c-jun转录和表达来实现。

Objective To observe the effect of paclitaxel on the transcription and expression of VSMC protooncogene. Methods The rat aorta muscle cell between the fourth and the ninth generation were used in the experiment. Reverse transcription-polymerase chain reaction and immunohistochemistry were used to detect the effect of angiotenson Ⅱ and paclitaxel of different concentration on the transcription and expression of SMC protooncogene c-fos and c-jun,respectively. Result High,medium and low dosage paclitaxel have no apparent effect on the transcription and protein synthesis of SMC protooncogene c-fos and c-jun. Conclusion Angiotenson Ⅱ promoted the proliferation by inducing the transcription and expression of c-fos and c-jun. The role of resisting proliferation of paclitaxel is not by the means of preventing the transcription and expression of c-fos and c-jun.