摘要
目的了解本院血液感染的细菌种类及耐药性。方法采用全自动血培养仪BACTEC9050进行培养,细菌鉴定采用ATB Expression系统,药敏试验采用K-B法。结果血液培养阳性率为10.16%,检出细菌23种共229株,其中革兰阳性细菌139株(60.70%),革兰阴性细菌76株(33.19%),真菌14株(6.11%)。革兰阳性细菌以表皮葡萄球菌(81/139)为主,革兰阴性菌中以大肠埃希菌(45/76)为主。表皮葡萄球菌对利福平、呋喃妥因、米诺环素、万古霉素和替考拉宁有较高敏感性。大肠埃希菌对哌拉西林/他唑巴坦、头孢吡肟、阿米卡星、美罗培南和亚胺培南有较高敏感性。结论本院血液标本细菌检出率较高,了解血液感染细菌的分布特征和耐药性,对减少院内感染,指导临床合理使用抗生素有重要意义。
Objective To investigate the kinds of bacteria and their drug resistance in blood cultures of our hospital. Methods Bacteria was detected with BACTEC - 9050 Autoanalyzer and ATB Expression. The K-B method was applied for the drug susceptibility test. Results The positive rate of blood cultures was 10.16% and 229 bacterial strains were detected. There were 139 strains of Grain-positive bacteria (60.70%) ,76 strains of Gram - negative bacteria (33.19%) and 14 strain of fungns(6.11% ). The main bacterial in Gram - positive bacteria was Staphylococcus epidermidis and in Gram-negative bacteria was Escherichia coll. Staphylococcus epidermidis were highly sensitive to rifampicin,nitrofurantoin,minocyeline, vaneomyein and teieoplanin, while Escherichia coli to piperacillin/tazobactam, cefepime, amikacin, imipenem and meropenem. Conclusion The isolated rote in blood culture was high and it was of great significance to decrease nosocomial infections and clinical chemotherapy by blood cultures.
出处
《南华大学学报(医学版)》
2008年第3期372-374,共3页
Journal of Nanhua University(Medical Edition)
关键词
血培养
细菌
耐药性
blood culture
bacteria
drug resistance