固定化IFN-γ和TNF-α对HeLa细胞凋亡线粒体膜电位及细胞周期影响的研究

INVESTIGATION OF MITOCHONDRIAL POTENTIAL AND CELL CYCLE IN HeLa CELL APOPTOSIS

利用光化学固定方法,分别以20 ng/mL和200 ng/mL 2种不同质量浓度将IFN-γ和TNF-α共同偶联到高分子材料聚苯乙烯基板上,合成光活性材料.分别设置正常对照组、游离组和共固定组,作用3 d,流式细胞术检测不同处理方式的细胞因子对人宫颈癌细胞线粒体膜电位及细胞周期的影响.结果表明共固定细胞因子能使HeLa细胞线粒体膜电位下降;细胞周期影响的分析进一步表明游离细胞因子主要诱导HeLa细胞在S期凋亡,而共固定化细胞因子诱导的凋亡则可能属于细胞周期非特异性诱导机制,提示共固定化细胞因子可能为肿瘤治疗开创新的领域.

The purpose of this article is to investigate the synergistic effects of IFN - γ and TNF - α on the apoptosis of the HeLa cell. Using the Photo - immobilization method, IFN - γ and TNF - α were coupled to the polystyrene cell culture plate with different concentration and synthesized the optical active material. The experiments were carried on natural and controlled groups respectively. Through the flow cytometry, the changes of HeLa cells were detected in 3 days. The results showed that ： the co - immobilization of the two cell factors has a stronger inhibition effect on the HeLa cell than natural IFN - γ and TNF - α. From the cell cycle, the mechanism of apoptosis between the co - immobilization cell factors and the natural one may be different.