血清胰岛素免疫检测及相关问题

Serum insulin immunoassays and relevant issues

血清胰岛素检测对糖代谢疾病的研究有着重要的临床参考价值。胰岛素检测技术经历了近50年的衍变历程，大体上能符合临床研究的要求。但由于胰岛素分子免疫原性和生物活性的多元性、检测体系的复杂性和多重因素的干扰性，目前仍缺乏统一的参考标准，给临床和不同实验室进行胰岛素测定值比较带来一定困难。

Serum insulin immunoassay plays an important role in the investigation of glucose metabolic disorders. Insulin assay has undergone method innovations for 50 years. Diverse insulin molecular immunogenicity and multiform biological activities, in addition to the immunoassay complexity, make serum insulin assays practically interfered by the factors such as blood sample qualities, serum anti-insulin antibodies and immunological cross-reactivity. Meanwhile, lack of standardization has imposed obstacles in the comparative studies among different reference laboratories.