细胞凋亡相关基因表达谱与大鼠再生肝的细胞凋亡关系

RELATIONSHIP BETWEEN EXPRESSIONAL PROFILES OF APOPTOSIS RELATED GENES AND APOPTOSIS ACTION OF THE RAT REGENERATING LIVER

目的在基因转录水平探讨细胞凋亡相关途径对大鼠肝再生的作用。方法采用大鼠2/3部分肝切除（PH）方法,制备再生肝模型,同时设对照手术（假手术）。用查阅网站资料和相关论文等方法获得凋亡相关基因,用大鼠基因230 2.0芯片检测它们在大鼠再生肝中的表达情况,通过比较真、假手术中上述基因的表达差异性确定肝再生相关基因。结果细胞凋亡相关基因中,252个基因与肝再生相关。在肝再生启动（PH后0.5～4h）、G0/G1过渡（PH后4～6h）、细胞增殖（PH后6～66h）、细胞分化和结构功能重建期（PH后72～168h）等4个阶段起始表达的基因数为81、231、55和16,总表达的基因数为161、100、733和192,表明相关基因主要在肝再生启动阶段起始表达,在不同阶段发挥作用。它们共上调795次,下调291次,表明肝再生中大部分基因表达增强。它们的表达模式分为35种,表明肝再生中细胞凋亡相关基因的表达情况多样和复杂。结论15条细胞凋亡途径参与肝再生调控。

Objective To investigate the actions of apoptotic pathways in rat liver regeneration （LR） at the transcription level. Methods The apoptosis genes were obtained by referring to the putative literatures and databases. Apoptotic-related genes expression profiles in rat LR were checked by Rat Genome 230 2.0 array. Identification of LR-associated genes was made by comparing the discrepancies of genes expression between partial hepatectomied （PH） and sham operatied （SO） rats. Results 252 genes were found to be related to liver regeneration. The initial and total expressing gene numbers occurring in the initiation phase of liver regeneration （0.5-4hours after PH）, G0/G1 transition （4-6hours after PH）, cell proliferation （6-66hours after PH）, cell differentiation and structure-function reconstruction （72-168hours after PH） were 81,23,155, 16 and 161,100,733,192 respectively. This demonstrated that LR-related genes primarily started their expression in the initiation phase, and worked in the different phases. The total times of their up- and down-regulated expression were 795 and 291, demonstrating that expression of most genes was enhanced in LR. Their expression patterns could be classified into 35 types, indicating the expressions of apoptosis-related genes in LR were diverse and complicated. Conclusion There are fifteen kinds of apoptosis pathways that associate to apoptosis regulation in the regenerating liver.