胃癌RUNX3表达对凋亡与增殖细胞转换的影响及其预后价值

EFFECTS OF RUNX3 ON CELL TURNOVER IN GASTRIC CANCER AND ITS PROGNOSTIC VALUE

目的探讨胃癌RUNX3基因表达对细胞凋亡与增殖细胞转换[凋亡率与增殖率之比值（AI/PI）]的影响及其与胃癌预后的关系。方法应用免疫组织化学分析RUNX3基因的表达;应用流式细胞术分析胃癌细胞的凋亡率和增殖率;采用Kaplan-Meier限乘法计算生存率。结果在60例胃癌中RUNX3基因的阳性表达率为46.7%。胃癌细胞RUNX3基因表达与淋巴转移和远处转移有关（P〈0.05）。胃癌细胞的AI/PI经频数分布表分析和检验均服从正态分布。胃癌细胞AI/PI范围显示为0.05～0.46,胃癌细胞AI/PI与淋巴转移和远处转移有关（P〈0.05）。胃癌细胞中RUNX3和AI/PI的表达之间存在明显相关关系（r=0.39,P〈0.05）。用Kaplan-Meier生存曲线经Logr-ank检验发现,RUNX3的阳性表达与生存相关（P〈0.05）。结论RUNX3基因可能通过影响凋亡与增殖细胞转换而影响胃癌的发生发展;RUNX3基因检测可作为胃癌临床病理学特点和预后的指标。

Objective To evaluate the relationship between the RUNX3 gene, ceil turnover（apoptosis and proliferation） and survival in gastric cancer patients. Methods RUNX3 was examined using immunohistochemical staining. Cell turnover was analyzed by flow cytometry using standard techniques. Survival was evaluated by Kaplan-Meier survival curves. Results The positive-expression rate of RUNX3 in tumor tissue from 60 patients with gastric cancer was 46.7% . RUNX3 positive-expression was correlated with lymph node metastasis and distant metastasis （P 〈 0.05）. Normal distribution was found by frequency distribution of AI/Pl in gastric carcinoma cells. Tumor cells exhibited a wide range of AI/PI expression from 0.05 to 0.46.Cell turnover was also correlated with lymph node metastasis and distant metastasis （ P 〈 0.05）. It was determined that the expression of RUNX3 and cell-turnover rates were associated （ r = 0.39, P 〈 0.05） . Using Kaplan-Meier survival curves and the Log-rank test, there was also a correlation between RUNX3 and survival （ P 〈 0.05 ）. Conclusion The RUNX3 gene may be related with tumorigenesis and tumor progression by affecting cell turnover, The detection of RUNX3 may be an important factor in evaluating the clinicopathological parameters and prognosis of gastric carcinoma.