弹性纤维参与大鼠心肌梗死后重构的形态学观察

MORPHOLOGICAL OBSERVATION ON ELASTIC FIBERS IN THE REMODELING MYOCARDIUM OF RATS AFTER INFARCTION

目的探讨大鼠心肌梗死重构过程中弹性纤维的变化及其机制和意义。方法SD大鼠冠状动脉前降支结扎形成心肌梗死模型,于术后2、4、8、12、21周时,用Masson染色、Uana地衣红染色技术和免疫组织化学方法,分别观察梗死区弹性纤维和白细胞介素1β（IL-1β）、肿瘤坏死因子（TNF-α）的变化,计算机图像分析系统作半定量分析。结果心肌梗死后2周梗死区即出现弹性纤维,至8～12周最高,21周弹性纤维明显减少或缺如,以胶原纤维为主。IL-1β、TNF-α表达在梗死后2周时可见,并随时间而增强。结论弹性纤维参与心肌梗死后的愈合过程,干预弹性纤维的形成可能成为抗心室重构的手段。

Objective To investigate the changes and role of elastic fibers during the progression of ventricular remodeling after myocardial infarction （MI） in rats. Methods Male Sprague Dawley rats were induced by ligating the anterior descending coronary artery and were divided into the 2nd, 4th, 8th, 12th and 21st week groups. Elastic fibers were evaluated in the infarct region using Masson trichrome staining, Uana orcein staining and image analysis. The expressions of TNFα and IL-1β were also detected by immunohistochemistry. Results Elastic fibers could be found within the infarct region in the 2nd week after MI, increased with time and reached the peak during the 8th to 12th weeks, decreased or were absent in the 21st week. The expressions of TNF-α and IL-1β were also detected in the infarct zone and increased with time. Conclusion Elastic fibers were involved in infarct healing and may play an important role in promoting ventricular remodeling.