双分子荧光互补技术

Bimolecular Fluorescence Complementation Technique

双分子荧光互补(bimolecular fluorescence complementation,BiFC)是近年发展起来的用于体内或体外检测蛋白质相互作用的一项新技术.该技术是将荧光蛋白在合适的位点切开形成不发荧光的2个片段,这2个片段借助融合于其上的目标蛋白的相互作用,彼此靠近,重新形成能具有活性的荧光蛋白.BiFC方法简单直观,既可以检测蛋白之间的相互作用,也可以定位相互作用蛋白质的位点.多色BiFC系统共用或与荧光共振能量转移(FRET)技术联用,还可以检测细胞内多个蛋白质的相互作用.

Biomolecular fluorescence complementation （BiFC） is a recently developed new technique to detect the protein-protein interactions in vitro and in vivo. The technique is based on the principle that two nonfluorescent fragments of a fluorescent protein dissected at appropriate site are brought together and reconstructed to fluorescence, depending on the association or interaction between the proteins fused to each fragment. BiFC method not only enables directly visualization and localization of protein interactions but also is simple and noninvasive. Moreover, multiple color BiFC systems or combing BiFC and fluorescence resonance energy transfer （FRET） technology may be exploited to detect the multiple protein-protein interactions.