重复肢体缺血对健康受试者全身血浆NO及NOS含量的影响

THE EFFECTS OF REPETITIVE LIMB ISCHEMIA ON THE SYSTEMIC CONCENTRATION OF NO,NOS IN PLASMA OF HEALTHY HUMANS

目的:研究人远程缺血预适应模型——重复肢体缺血(RLI)对健康成人血浆一氧化氮(NO)和不同类型NO合酶(NOS)含量的影响。方法:血计袖带压迫非优势侧肱动脉(压力200mmHg,缺血/放松5min/5min,共5次),持续监测生理指标,记录主观感受。在Pre、Post-0h、Post-4h和Post-24h抽取压迫对侧肘静脉血。应用Griess法检测NO含量,用化学法检测NOS含量。结果:受试者血压、心率在正常范围内波动。压迫侧肢体的不适感随着压迫次数逐渐减轻。血浆NO和iNOS在Post-0h、Post-4h和Post-24h较Pre有显著提高(P<0.05)。总NOS在Post-0h和Post-4h较Pre有显著提高(P<0.05)。而cNOS在RLI后各时间点与Pre比较无统计学差异(P>0.05)。结论:RLI是安全,可耐受的方法,其可诱导健康人血浆NO及总NOS、iNOS含量升高,并至少维持4～24h。

Aim： To investigate the effects of RLI on plasma nitric oxide（NO） and NO synthase（NOS） isoforms of healthy humans, Methods： 30 healthy human subjects（aged from 40-70 years old） were recruited, RLI was induced by five 5 min cycles of ischemia of non-dominant arm（200 mmHg, 5 min interval）, Blood pressure, heart rate, and the feelings of ischemic arm were continuously mo nitored. Venous plasma was collected in contralateral arm at Pre, Post-0 h, Post-4 h, and Post-24 h, Plasma level of NO was measured by Griess reaction, and NOS was measured by chemical method. Results： Blood pressure and heart rate varied in normal range. The uncomfortable feeling was decreased with the increasing numbers of ischemie cycles, Plasma level of NO, and iNOS in plasma were significantly increased at Post-0 h, Post-4 h, and Post-24 h compared to Pre（P 〈 0.05）. tNOS was also significantly increased at Post-0 h and Post-4 h compared to Pre （ P 〈 0.05）. No significant change in plasma cNOS was shown at following three time points than Pre, Conclusion： These findings suggest that RLI can elevate plasma level of NO, tNOS, and iNOS in healthy humans, RLI might be a safe method as a rlPC, and it would have important possibility to be performed in clinic.