人类正常胃肠道组织基因表达谱特征的生物信息学分析

GENE EXPRESSION PROFILE OF HUMAN NORMAL GASTROINTESTINAL TRACT TISSUES:BIOINFORMATIC STUDY

目的:筛选人类正常胃肠道组织特异性高表达基因。方法:以公开发表的人类正常组织基因表达数据库资料为研究对象,应用单侧StudentT检验筛选胃、回肠和结肠组织的特异性高表达基因。Ingenuity软件和KEGG分析特异性高表达基因相关的生理功能;Cluster软件分析胃特异性高表达基因谱在胃癌基因数据库中的表达特征。结果:筛选出胃高表达基因196个,回肠高表达基因203个,结肠高表达基因224个,高表达基因的功能与胃肠道的主要生理功能相吻合。筛选出的正常胃肠高表达基因中含有一些癌基因和抑癌基因。系统聚类分析发现胃特异性高表达基因在胃癌数据库的胃正常组织中高表达,而相应的胃癌组织低表达,癌组织和正常组织可以完好聚类分组,并且可以区分中分化和低分化胃癌。结论:人类正常胃、回肠和结肠具有组织特异的与生理功能密切相关的高表达基因群。

Aim： To identify up-regulated genes specific to human normal gastrointestinal tissues. Methods： Study was made on human normal tissue gene expression database open to the public. Tissue-specific genes were identified using one-tailed student T test. Online software including Ingenuity and KEGG were applied for physiological function analyses. Unsupervised two-way hierarchical clustering method was used to analyze the expression profile of stomach-specific genes in gastric cancer gene expression datasets. Results： The analyses identified 196 stomach-specific genes, 203 ileum-specific genes and 224 colon-specific genes, respectively. The gene expression profiles reflect major organ-specific physiological functions on the molecular level. Some putative oncogenes and tumor suppressor genes were found in the tissue-specific gene list. Hierarchical clustering analysis revealed that the stomach-specific genes were up-regulated in normal stomach tissues but down-regulated in stomach cancer tissues. The normal tissues clustered together, so did the cancer tissues. At the meantime, clustering could also distinguish the moderate and severe differentiated stomach cancer. Conclusion： Human normal stomach, ileum and colon possess tissue-specific up-regulated genes, which are closely associated with physiological functions.