耳蜗外毛细胞的几种分离方法的比较及形态学观察

COMPARISONS OF SEVERAL METHODS USED TO ISOLATE OUTER HAIR CELLS AND MORPHOLOGICAL OBSERVATIONS IN COCHLEA

目的:比较几种耳蜗外毛细胞的分离方法及其形态学观察。方法:分别采用三种方法分离耳蜗外毛细胞,并进行了光镜下及耳蜗铺片硝酸银染色下的形态学观察。结果:三种分离方法均可分离出活性良好的耳蜗外毛细胞(OHC);耳蜗铺片硝酸银染色观察到耳蜗外毛细胞的形态和分布状况。结论:成功地分离出单离的活性良好的耳蜗外毛细胞,这对于深入研究它们的正常生理功能及某些病理状态下的功能及形态变化意义重大。

Aim： The comparison of several methods which were used to isolate cochlear outer hair cells and the observations of morphology were researched. Methods： Three different separating methods of outer hair cells in cochlea were adopted; the morphology of outer hair cells in cochlea and the morphology of cochlear stretched preparation in Silver Nitrate staining were also investigated. Results： Single alive OHC in cochlea was disassociated by all methods, with microscope and cochlear stretched preparation＇s staining. We could also observe appearances and distributions of OHC in cochlea. Conclusion： It is successful to isolate single alive cochlear OHC, and it will be very important to investigate deeply normal physiological functions and changes of functions and morphology in some pathologic status in cochlear OHCs.