MBP刺激下IL-3和IL-4对树突状细胞前体定向分化的影响

Effect of IL-3,IL-4 on the Directional Differentiation of Dendritic Cells by the Stimulation of Myelin Basic Protein

目的观察在髓鞘碱性蛋白(myelin basic protein,MBP)刺激下,不同细胞因子对树突状细胞(dentriticcell,DC)前体定向分化的影响。方法分离实验性自身免疫性脑脊髓炎(EAE)易感性BALB/c小鼠脾脏DC,在体外不同培养条件下与MBP共培养。用流式细胞仪检测DC荧光抗体标记的CD11c和CD8α表达。结果(1)在MBP和粒巨嗜细胞集落刺激因子(GM-CSF)培养条件下,白细胞介素-4(IL-4)能明显刺激DC CD11c表达,IL-4组其平均荧光强度为856.93±12.45,对照组为708.58±21.25,两组比较差异有统计学意义(P<0.01);(2)在GM-CSF和MBP培养条件下,IL-3能明显上调DC CD8α的表达,IL-3组平均荧光强度为7 751.70±296.63,对照组为7 279.17±176.77,两组比较差异有统计学意义(P<0.01)。结论IL-4可进一步促进BALB/c小鼠脾脏DC前体向DC1分化,而IL-3则促进DC前体向DC2分化。

Objective To observe the influences of different cytokines on the directional differentiation of dentritic cells （DC） by the stimulation of myelin basic protein （MBP）. Methods DCs were separated from the spleen of BALB/c mice and co-cultured with MBP in witro with different cytokines, The fluorescence antibodies CD11c and CD8α which were the notation antigens of anti-DC1 and anti-DC2 were exerted to mark the cells. The result was detected by flow cytometer. Results （1）Co-cultured with GM-CSF and MBP, IL-4 could stimulate DC precursor to express extremely more CDllc. The mean fluorescence intensity of CDIIc of the study group （856.93±12.45） was significantly higher than that of the control （708.58±21.25）（P〈0.01）. （2）Co-cultured with GM -CSF and MBP, IL-3 could op-regulate the expression of CD8α of DCs. The mean fluorescence intensity of CD8α of the study group （7751.70± 296.63） was extremely higher than that of the control （7279.17 176.77） （P〈0.01）. Conclusions IL-4 could further promote splenic DC precursor differentiation into DC1 in BALB/c mice, while IL-3 could promote DC precursor to DC2.