黑芝麻不同外植体离体培养研究

StudyontheCulture ofDifferentExplantsof BlackSeedcoat SeameinVitro

以黑芝麻无菌苗的下胚轴、幼根、子叶为外植体，以ＭＳ＋２，４－Ｄ１．５ｍｇ／Ｌ＋ＺＴ０．５ｍｇ／Ｌ为愈伤组织诱导培养基；ＭＳ＋６－ＢＡ２ｍｇ／Ｌ－ＩＡＡ０．５ｍｇ／Ｌ为分化培养基；１／２ＭＳ＋０．５ｍｇ／ＬＮＡＡ＋ＩＡＡ０．２５ｍｇ／Ｌ为生根培养基进行培养。结果表明，幼根能产生少量愈伤组织，尚未分化出苗；子叶愈伤组织能分化出苗，但分化率低；下胚轴愈伤组织不仅可分化出苗（分化率为３６％），而且可形成大量具有分化潜力的胚性愈伤组织，分化绿苗的频率可达６７％。

Explants were hypocotyls , young roots , cotyledons of aseptic seedlings of blackseedcoat seame。Inducedmediumfor calliwas MSmediumwith2,4- D1.5mg/LandZ T0.5mg/L。Differentiation mediumforbudwas MSmediumwith6- BA2mg/LandIAA0.5 mg/L。Inducedmediumfor root was1/2MSmedium withNAA0.5mg/LandIAA0.25 mg/L;Theresultsindicated:afew calliformedfrom young rootshvitro,butthere werenobudsformedinculture。Somebudsweredifferentiatedfrom calliofcotyledonsCallifromhypocolylsnotonlydifferentiatedtosomebudsbutalsoformedalotofembryoniccalliwhichhaddifferentiation capacity. The differentiation ratio of green shoots could reach to 43%。 Hypoco1ylswerethebestexplantsinthecultureofblackseedcoatseameinvitro。