防治褐飞虱的高毒农药替代药剂的室内筛选及交互抗性研究

Laboratory Screening and Cross-Resistance Analysis of Alternative Insecticides for Highly-Toxic Pesticides for Controlling Brown Planthopper,Nilaparvata lugens

为了筛选防治褐飞虱的高毒农药的替代药剂,于2005-2006年,采用稻茎浸渍法测定了6类20余种杀虫剂对广西南宁、桂林,湖南常德和江苏南京褐飞虱种群的室内毒力。结果表明,噻嗪酮、氟虫腈、噻虫嗪、烯啶虫胺、毒死蜱、异丙威、猛杀威、丁硫克百威等8种药剂对褐飞虱具有较高的毒力,可作为替代高毒药剂的候选品种。采用稻茎浸渍法对1个室内褐飞虱种群用吡虫啉筛选23代后,褐飞虱对吡虫啉的抗性从筛选前的200.1倍上升至筛选后的1298.5倍(上升了5.5倍);高抗吡虫啉的褐飞虱种群对氯噻啉、噻虫啉和啶虫脒表现出明显的交互抗性,而对呋虫胺、噻虫嗪和烯啶虫胺则无明显的交互抗性。还讨论了褐飞虱的抗性治理策略。

To screen alternative insecticides for highly-toxic pesticides, toxicities of more than twenty insecticides fallen in six groups to the 3rd instar nymphs of brown planthopper, Nilaparvata lugens populations collected from Nanning and Guilin Cities （Guangxi Zhuang Autonomous Region）, Changde City （Hunan Province） and Nanjing City （Jiangsu Province）, China were investigated by the rice stem-dipping method in laboratory from 2005 to 2006. Results showed that buprofezin, fipronil, thiamethoxam, nitenpyram, chlopyrifos, isoprocarb, promecarb and carbosulfan could be adopted as potential alternative insecticides for the highly-toxic pesticides. After selection with imidacloprid for 23 generations, the population developed extremely-high level resistance to imidacloprid （1298.5-fold）, with a LCso value of 103.88 mg/L, being 6.5-fold of the value of the original reference strain before the selection （16.01 mg/L）. The selected strain of N. lugens showed obvious crossresistance to. imidaclothiz, thiacloprid and acetamiprid, whereas not to dinotefuran, thiamethoxam and nitenpyram. Management strategy of insecticide resistance of the insect was also discussed.