琼脂糖凝胶电泳在水稻EcoTILLING技术中的应用

Application of Agarose Gel Electrophoresis in Rice EcoTILLING

为构建低成本的基于琼脂糖凝胶电泳的EcoTILLING技术体系,用于水稻及其他生物自然群体变异的检测,对基于琼脂糖凝胶的EcoTILLING技术进行了优化。结果表明,从芹菜中提取的CELⅠ核酸酶粗提物结合琼脂糖凝胶电泳检测技术,可以成功地检测到目的基因位点的突变。利用该技术,在云南地方水稻品种大吊糯中检测到2个SNPs。

To establish a cheaper EcoTILLING system based on agarose-gel electrophoresis for the detection of natural variation of rice or other plant species, a convenient EcoTILLING technique combined agarose gel electrophoresis with active rude CELI nuclease extract from celery was constructed. By using the EcoTILLING technique, two SNPs were detected in a Yunnan conventional rice variety Dadiaonuo.