摘要
以粪鬼伞菌丝体为原料,采用超声波辅助浸提法提取鬼伞菌多糖(CPS),并利用响应面分析法及正交实验分析法优化提取工艺,在超声波功率为500w、超声波时间为20min,料液比1∶80、水浴提取温度为80℃、提取时间为1.5h时,CPS的提取率达到最大;经气相色谱分析,CPS由木糖、葡萄糖、半乳糖和鼠李糖4种单糖组成,摩尔比为木糖∶葡萄糖∶半乳糖∶鼠李糖=1.38∶12.85∶4.81∶1.00。
Ultrasonic-assisted technology was used to extract polysaccharide from submerged-cultured mycelium of Coprinus sterqulinus Fr. (CPS). The response surface and orthogonal design analysis were used to optimize the process conditions. At ultrasonic power 500w for 20 min, solvent-to-sample of 80:1, water bath at 80℃ for 1.5h, the extraction rate of CPS was the biggest. CPS contained xylose, glucose, galactose and mannose at the rate of 1.38:12.85:4.81:1.00, which analyzed by GC.
出处
《中国食用菌》
2008年第5期38-40,共3页
Edible Fungi of China
基金
吉林省教育厅科技计划项目
合同编号:200660
关键词
粪鬼伞多糖
提取工艺
单糖组成
CPS
Extraction process
Monosaccharide constitution