孕激素受体、17β羟类固醇脱氢酶与子宫内膜异位症的关系

Correlation of progesterone receptor gene and 17β hydroxysteroid dehydrogenase with endometriosis

目的探讨孕激素受体(PR)、17β羟类固醇脱氢酶(17βHSD)在子宫内膜异位症(内异症)在位与异位内膜的表达与发病的关系。方法2005年6月至2006年10月在中国医科大学盛京医院和解放军463医院采集内异症(内异症组)38例和宫颈上皮内瘤样病变(对照组)25例行子宫切除的分泌期子宫内膜,内异症患者配对卵巢巧克力囊肿组织作为研究标本。Western印迹杂交检测PRA与PRB蛋白表达,RT-PCR检测17βHSD2mRNA表达。结果内异症组在位内膜PRA蛋白相对表达量70.79±10.82,对照组78.99±16.02(P>0.05);内异症组在位内膜PRB蛋白相对表达量64.87±10.10,对照组156.13±21.46,内异症组PRB蛋白表达显著低于对照组(P<0.01);内异症组卵巢异位内膜PRA蛋白相对表达量67.04±6.90,配对在位内膜70.79±10.82(P>0.05);卵巢异位内膜PRB蛋白相对表达量60.75±6.73,配对在位内膜64.87±10.10(P>0.05)。内异症组在位内膜17βHSD2mRNA表达量1.04±0.11,对照组1.10±0.16(P>0.05);卵巢异位内膜17βHSD2mRNA表达量0.17±0.02,配对在位内膜1.04±0.11,异位内膜低于配对在位内膜(P<0.05)。结论内异症患者在位内膜和异位内膜PRB阴性表达与内异症发病有关;内异症患者异位内膜缺乏17βHSD2与内异症发病有关。

Objective To investigate whether there are differential expression of progesterone receptor gene （PRA and PRB） and 17β hydroxysteroid dehydrogenase type2（ 17βHSD2 ） between eutopic endometrium and its diseased counterpart endometriotic tissue. Methods From June 2005 to Oct. 2006, eutopic endometrium and ovarian endometriosis were obtained from the patients with endometriosis, who were from China Medical University Shengjing Hospital and PLA 463 Hospital, and the controls, the patients with cervical intraepithelial neoplasia. The expression of PRA and PRB was examined by western blot and 17βHSD2 by RT-PCR. Results The level of PRA proteins were 70.79 ± 10.82 in eutopic endometrium of endometriosis and 78.99 ± 16.02 in controls respectively （ P 〉 0. 05 ）. The expression of PRB proteins was significantly lower in endometriosis （64. 87 ± 10. 10） than control（ 156. 13 ± 21.46）. There was a significant difference beteewn them （ P 〈 0.01 ）. The level of PRA and PRB proteins was 67.04 ± 6.90 and 60.75 ± 6.73 in ovarian endometriotic tissue and 70.79 ± 10.82 and 64.87 ± 10. 10 in paired eutopic endometrium of endometriosis （ P 〉 0.05 ）. The level of 17βHSD2 mRNA was 1.04 ± 0. 11 and 1.10 ± 0.16 in eutopic endometrium of endometriosis and control （P〉 0. 05 ）. 17βHSD2 mRNA was significantly lower in ovarian endometriotic tissue（ 0. 17 ± 0.02） than control （ 1.04 ± 0.11 ） （ P 〈 0.05 ）. Conclusion The absence of PRB in eutopic endometrium and endometriotic tissue of endometriosis and 17βHSD2 in endometriotic tissue may be associated with endometriosis.