利用叶绿素荧光预测水蜜桃果实冷害的研究

Chlorophyll Fluorescence as a Tool to Predict Formation of Chilling Injury of Peaches in Storage

采用叶绿素荧光技术检测冷藏条件下水蜜桃冷害发生进程中初始荧光(Fo)、最大荧光(Fm)、可变荧光(Fv=Fm-Fo)、光化学效率(Fv/Fm)等叶绿素荧光动力学参数的变化,分析了叶绿素荧光参数与果肉出汁率的相关性以及与呼吸作用的关系。研究结果表明,在冷藏过程中,叶绿素荧光参数都呈下降趋势,冷害发生以后,Fm和Fv/Fm下降速度加快,而中途加温处理能够缓解Fm和Fv/Fm的降低。同时,Fm和Fv/Fm与果肉出汁率之间存在显著的正相关,利用测定Fm和Fv/Fm能够初步预测冷害的发生情况。

Chlorophyll fluorescence parameters （Fo, Fm and Fv/Fm） were measured by Portable Chlorophyll Fluorometer when peaches [ Prunus persica （L.） Batsch ] were stored at 0℃. Extractabe juice and respiration rate of peaches were also measured during the period of storage. At last, we assessed the relationships among chlorophyll fluorescence parameters, respiration rate and storage time. The results showed that all chlorophyll fluorescence parameters declined with the progress of fruit ripening, where Fm and Fv/Fm declined more quickly when chilling injury happened. But intermittent warming at 20℃ for 36 h after 10 days storage can prevent Fm and Fv/Fm decline. Fm and Fv/Fm showed the best correlation with changes of extraetabe juice. This suggested a relationship between the fluorescence and formation of chilling injury. Chlorophyll fluorescence as a tool may predict formation of chilling injury of peaches in storage.