摘要
目的:观察中药提取物槲皮素对食管癌细胞侵袭的影响,并探讨其可能机制.方法:采用不同浓度的槲皮素处理食管癌EC109细胞后,以软琼脂集落培养试验检测癌细胞锚着不依赖性增殖,以Boyden小室模型方法检测癌细胞侵袭能力,采用荧光实时定量RT-PCR检测癌细胞中期因子(midkine,MK)基因mRNA水平,以Western blot方法检测癌细胞MK基因蛋白水平变化.结果:食管癌EC109细胞经槲皮素处理后,恶性增殖和侵袭能力均明显下降,且呈剂量依赖性(均P<0.05).槲皮素处理组MK基因mRNA和蛋白水平均明显下调,且呈时间和浓度依赖性(均P<0.05).结论:槲皮素可明显抑制食管癌EC109细胞侵袭能力,其机制可能与下调MK基因表达有关.
AIM: To observe the effects of Quercetin on the invasion of human esophageal cancer cells, and to investigate the possible mechanism. METHODS: Human esophageal cancer cell line EC109 was treated with different concentrations of Quercetin, and then the anchorage independence growth of EC109 cells was studied by colony formation in soft agar; the invasion ability of EC109 cells was determined by Boyden chamber, and the expression levels of midkine mRNA and protein were detected by real time RT-PCR and Western blot assay, respectively. RESULTS: After Quercetin treatment, the malignant proliferation and invasive ability of EC109 cells were obviously decreased, which showed a dose-dependent manner (all P 〈 0.05). Meanwhile, Quercetin down-regulated the expression of midkine mRNA and protein in a dose- and time-dependent manner (all P 〈 0.05). CONCLUSION: Quercetin can inhibit the invasion of EC109 cells by down-regulating midkine expression.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第22期2503-2506,共4页
World Chinese Journal of Digestology
基金
中国博士后基金资助项目
No.2003033547~~
关键词
食管肿瘤
槲皮素
侵袭
中期生长因子
免疫印迹
逆转录-聚合酶链反应
Esophageal carcinoma
Quercetin
Invasion
Midkine
Western blot
Reverse transcription-polymerase chain reaction