改良双袖套法大鼠原位肝移植500例

Othotopic liver transplantation in 500 rats using a reformed two-cuff method

目的:改进大鼠肝移植的方法,缩短无肝期,提高手术成功率,总结大鼠原位肝移植经验.方法:正式实验分组:(1)预输注供者凋亡的脾细胞正常大鼠肝移植研究组(分4小组);(2)同期输注供者凋亡的脾细胞对正常大鼠肝移植研究组(分4小组);(3)术后输注供者凋亡的脾细胞正常大鼠肝移植研究组(分4小组);(4)预输注供者凋亡的脾细胞对肝硬化大鼠肝移植研究组(分4小组);(5)预输注供者凋亡的血液淋巴细胞对正常大鼠肝移植研究组(分3小组),每小组各10只大鼠.观察手术时间以及大鼠肝移植2d和1wk存活率.结果:在正式实验大鼠肝移植中,供体手术时间30±5min,供肝热缺血时间2±0.5min,袖套准备及肝脏修整时间10±2min,受体手术时间51±10min;无肝期16±4min.冷缺血时间61±5min.正式实验大鼠肝移植2d存活率96.8%(184/190),1wk存活率95.3%(181/190).结论:改进显露方法及肝上下腔静脉吻合方法后,手术简化,同时并发症减少,生存率提高.

shorten anhepatic phase, and to raise the success rate of operation. METHODS： The animals used in formal trial were divided five groups： （1） liver transplantation on normal rats was carried out after infusing apoptotic splenocytes of donors 1 wk before transplantation （including 4 subgroups）; （2） liver transplantation on normal rats was carried out with simultaneous injection of apoptotic splenocytes from donors （including）; （3） apoptotic splenocytes of donors were infused after liver transplantation on normal rats was carried out （including 4 subgroups）; （4） liver transplantation on cirrhotic rats was carried out after infusing apoptotic splenocytes of donors 1 wk before transplantation （including 4 subgroups）; （5） liver transplantation on normal rats was carried out after infusing apoptotic lymphocytes of donors 1 wk before transplantation （including 3 subgroups）. Each subgroup contained 10 rats. Operating time, 2-day and 1-week survival rate of rats were observed. RESULTS： The operating time on donor rats, the warm ischemia time of donor livers, the time for cuff preparation, the operating time on recipient rats, the anhepatic phase and the cold ischemia time of donor livers were 30 ± 5 min, 2 ± 0.5 min, 10 ± 2 min, 51 ± 10 min, 16 ± 4 min and 61 ± 5 min, respectively, in formal trials. The 2-day and 1-week survival rates were 96.8% （184/190） and 95.3% （181/190）, respectively. CONCLUSION： The reformed two-method can simplify the operating procedure, reduce complication and raise the survival rates.