延边地区朝鲜族乙型肝炎病毒感染者病毒基因型的检测与分析

Inspection and analysis of hepatitis B virus genotypes among the korean nationality of Yanbian area in China

目的:分析延边地区朝鲜族HBV感染者HBV基因类型分布及其临床意义.方法:延边地区朝鲜族HBV感染者215例分为无症状携带者36例,急性乙型肝炎5例,慢性乙型肝炎106例,肝炎肝硬化50例,原发性肝癌18例.利用特异性引物聚合酶链反应法(PCR),对患者血清标本进行基因型检测.结果:215例HBV感染者中检出B基因型61例(28.0%),C基因型154例(72.0%),未检出其他基因型.在慢性乙型肝炎、肝炎肝硬化、肝细胞癌患者中,C基因型比例(79.0%、84.0%、89.0%)明显高于无症状携带者及急性乙型肝炎(25.0%、60.0%,P<0.01),在无症状携带者中,B基因型比例高于C基因型(75.0%vs25.0%,P<0.01).HbeAg阳性组C基因型比例高于HBeAg阴性感染组(73.0%vs54.0%P<0.05),HbeAg阴性组的B基因型比例高于HBeAg阳性组(46.0%vs27.0%,P<0.01).结论:延边地区朝鲜族HBV感染主要为C基因型,其次为B基因型.C基因型在临床上以慢性乙型肝炎、肝炎肝硬化、肝细胞癌多见,其发生率显著高于B基因型.

AIM： To investigate the distribution and clinical significance of hepatitis B virus （HBV） genotypes among the Korean Chinese of Yanbian area in China. METHODS： A total of 215 Korean Chinese with HBV infection in Yanbian area included symptomatic carrier （SC, n = 36）, acute hepatitis B （AHB, n = 5）, chronic hepatitis B （CHB, n = 106）, hepatitis-induced cirrhosis （HC, n = 50）, and primary hepatic carcinoma （HCC, n = 18）. Specific primer-polymerase chain reaction was used to detect the HBV genotypes in this cases. RESULTS： Of the 215 cases, genotype B and C were found in 61 （28.0%） and 154 （72.0%） cases, respectively. The ratio of genotype C was markedly higher in CHB （79.0%）, HC （84.0%）, HCC （89.0%） than that in SC （25.0%） and AHB （60.0%）, respectively （P 〈 0.05）. In SC, the proportion of genotype B was significantly higher than that of genotype C （75.0% vs 25.0%, P 〈 0.01）. The rate of genotype C was higher in the HBeAgpositive cases than that in the HbeAg-negative cases （73.0% vs 54.0%, P 〈 0.05）, but the rate of genotype B was the other way around （46.0% vs 27.0%, P 〈 0.01）. CONCLUSION： Genotype C HBV infection is predominant in the Korean Chinese of Yanbian area in China, and genotype B is the second. Genotype C is mostly found in CHB, HC and HCC.