IL-1A+4845-Fnu4H I基因多态性与慢性牙周炎易感性关系的研究

The association studies of IL-1 A+4845 genes polymorphisms with chronic periodontitis

目的：探讨中国汉族人IL-1A＋4845-Fnu4HⅠ基因多态性与慢性牙周炎易感性的关系。方法：收集66例中重度慢性牙周炎（CP）病人，50例健康对照者的静脉血，提取外周静脉血白细胞DNA，采用PCR—RFLP方法，检测IL-1A＋4845-Fnu4HⅠ基因型，比较两组受试者等位基因型检出率的差别。结果：CP组IL-1A＋4845-Fnu4HⅠ等位基因2的检出率和健康对照组无显著性差异（OR=1．05；95％CI=0．38～2．84；P〉0．05）。结论：IL-1A＋4845-Fnu4HⅠ等位基因2可能与汉族中重度慢性牙周炎遗传易感性无关。

AIM： To study whether the specific IL - 1 A ＋4845/Fnu4H Ⅰ genotype alleles were associated with CP in Chinese Han nationality. METHODS： CP group consisted of 66 patients and healthy controls group consisted of 50 subjects. Anti - coagulated peripheral blood sample was obtained from each subject and genomie DNA was extracted from each sample. Single nueleotide polymorphisms （SNPs） at IL - 1A ＋ 4845/Fnu4H Ⅰ were analyzed by standard polymerase chain reaction- restriction fragment length polymorphisms （PCR- RFLP） assay. RESULTS. Statistical analysis revealed no significant difference between the two groups. （ OR = 1.05 95% CI = 0. 38 - 2.84 P = 1. 000 P 〉 0. 05 ） . CONCLUSION： There is no evidence in the study supporting the association between single nueleotide polymorphisms （SNPs） of the IL- 1A ＋ 4845/Fnu4H Ⅰ gene and prevalence and/or severity of chronic periodontitis.