73例慢性乙型肝炎患者血清Th1/Th2型细胞因子水平研究

Research on serum level of Thl/Th2 cytokines in 73 patients with chronic hepatitis B

目的探讨慢性乙型肝炎患者血清中Thl/Th2型细胞因子水平与病情轻重、活动程度的关系。方法采用双抗体夹心酶联免疫吸附法(DAS-ELISA)检测73例不同病情程度、不同丙氨酸氨基转移酶(ALT)水平的慢性乙型肝炎患者血清Thl/Th2型细胞因子的水平。结果慢性乙型肝炎患者血清中3种Thl型细胞因子白细胞介素(IL)-2、干扰素(IFN)-γ、IL-12水平普遍较低,除轻、中度组与肝硬化组患者IFN-γ水平差异有统计学意义外,其余各组间差异无统计学意义。3种Th2型细胞因子IL-4、IL-6、IL-10水平各组间差异均无统计学意义,其中IL-6水平普遍较高,慢性重型肝炎组和肝硬化组患者血清IL-4和IL-10水平相对较高。患者6种细胞因子水平与ALT值均呈正相关。结论慢性乙型肝炎患者血清Thl型细胞因子IL-2、IFN-γ、IL-12和Th2型细胞因子IL-4、IL-6、IL-l0水平可反映其病情活动程度更甚于病情轻重程度。

Objective To explore the relationship between serum levels of Th1/Th2 cytokines of patients with chronic hepatitis B at different states and the activity degree of chronic hepatitis B. Methods The levels of three kinds of Thl type cytokines including interleukin（IL）-2, interferon（IFN）-γ, IL-12 and Th2 type cytokines including IL4, IL-6 and IL-10 of 73 patients with chronic hepatitis B were measured with double antibody sand- wich enzyme linked immunosorbent assay（DAS-ELISA）. Results The serum levels of IL-2, IFN-γand IL-12 of patients with chronic hepatitis B were generally lower, while it didn＇t have significantly difference between different groups; The levels of three kinds of Th2 cytokines including IL-4, IL-6 and IL-10 had no significant differences between different groups either, and the IL-6 level of patients was generally higher. The serum IL-4 and IL-10 levels of patients in chronic severe hepatitis group and hepatocirrhosis group were comparatively higher. It was found that levels of the six kind of cytokines of patients with chronic hepatitis B had positive correlation with ALT level of the patients. Conclusions The serum levels of Thl cytokines including IL-2, IFN-γ,IL-12 and Th2 cytokines including IL4, IL-6 and IL-10 of patients with chronic hepatitis B may reflect the activity of chronic hepatitis B more than the state of the disease.