气流动力学影响的小鼠气管移植模型的建立

A tracheal transplantation model for studying the impact of ventilation

目的建立并改进同种异体小鼠气管原位移植模型,用于研究气流动力学对供体气管的影响。方法C57BL/6小鼠气管为供体,移植于BALB/c小鼠。取两个供者气管各7个软骨环,两端的膜部分别予以侧侧缝合作并行连接,新建的气管段以原位移植的方式,移植于受体BALB/c小鼠。上述一供体气管用作小鼠的通气气管段,另一以4号线结封堵以阻断气流通过。28 d后获取标本,HE染色评价气道上皮的完整性、分化程度、黏膜下炎症细胞浸润和纤维组织增生情况。结果建立小鼠气管原位通气移植模型30例,从标本采集至移植完毕平均用时(100±14)m in。全组受体动物术后生存率90%,无感染。手术后28 d移植气管病理检查提示,存在通气的供体气管管腔通畅,假复层柱状纤毛上皮较连续并完整,黏膜下轻度炎细胞浸润和纤维组织增生。而阻断了气流通过的供体气管表现为扁平上皮分布,纤毛上皮减少,黏膜下纤维组织增生、淋巴细胞浸润明显。结论新建的小鼠气管移植模型可用于研究移植后的气流动力学影响,同时可用于观察受体气道上皮移行的动力学参数。

Objective To establish and modify tracheal transplantation model of allograft in order to study the impact of ventilation on transplants. Methods Two donor C57BL/6 tracheas, composed of seven rings each, were transplanted orthotopically into recipient BALB/c mouse. These two donor tracheas were sutured at the membrane at each end. Ventilation was maintained in one of the donor traehea, while blocked in the other by placing a piece of #4 suture in the lumen. The allografts were harvested on postoperative 28th day, and examined by HE staining, The histological appearances of the two pieces of allografts were compared, including the integrity and differentiation of epithelium, submucous lymphocyte infiltration, and fibroproliferation. Results Thirty transplantations were accomplished. The average operative time for each case was （ 100 ± 14） minutes. Survival rate was 90%, and no infection was observed. The histology suggested that the epithelium and luminal integrity were minimally impaired in allograft with normal ventilation, and was associated with minor lympho-infiltration and fibroproliferation. It was otherwise more severely impaired in the allografi without ventilation. Conclusion The presently established tracheal transplantation model can be applied to study the impact of ventilation on transplants ,and recipient＇s epithelium migration dynamics as well.