花生DNA导入大豆后代几种生化性状的变异分析

Analysis of Biochemical Variation of Soybean Progeny Transformed with Peanut DNA

分析了花生总ＤＮＡ导入大豆后，后代几种生化性状的变异表现。结果表明，变异后代种子的蛋白质含量明显提高，表现出超亲现象；脂肪含量有一定程度下降，低于双亲；氨基酸的组成也发生了变化，其总量高于双亲。变异后代过氧化物酶活力显著高于双亲，淀粉酶和超氧物歧化酶活力介于双亲之间。同工酶分析结果表明，变异后代的过氧化物酶、淀粉酶和超氧物歧化酶酶谱与亲本之间均有差异，且表现出与双亲明显不同的特征来，有的酶带明显源于供体亲本。

Biochemical variation of soybean (Glycine mex L.) progeny transformed with peanut (Arachis hypogaea L.) DNA was investigated. The protein contents of transgenic progeny significantly increase and were higher than those of their parents. Variations of amino acid components were detected between the parents and the transgenic progeny, and the total amount of amino acid of progeny was higher than that of its parents. The activity of peroxidase in progeny was significantly higher than that in its parents and the activity of amylase and superoxide dismutase in progeny were between their parents. The isoenzyme pattens of peroxidase, amylase and superoxide dismulase showed differences among the parents and progeny; several bands in the progeny were not present in the soybean recipient but were similar to those in the peanutal donors.