摘要
本文用发根农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｒｈｉｚｏｇｅｎｅｓ）ＰＲｉ９４０２对桔梗（Ｐｇｒａｎｄｉｆｌｏｒｕｍ）进行了感染实验，获得了发状根及其再生植株。它们多具有Ｒｉ质粒转化体的典型特征，并检测到农杆碱和甘露碱。筛选出一个增殖率高的发状根无性系（Ｐ９Ｃ３）和具有优良农艺性状的再生植株。
Hairy roots and regenerants of P.grandiflorum were obtained by infection with Agrobacterium rhizogenes PRi9402. Results showed that typical transformat phenotype and were detected Agropine and Mannopine. Hairy root clone (P_9C_3) of fast growth and regenerants of good agricultural characteristics were screened.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
1997年第4期52-55,共4页
Journal of Jilin Agricultural University
关键词
转化体多样性
桔梗
中药
PRi9402
transformation
divergence of transformats
P.grandiflorum
