摘要
将感染弓形虫速殖子的SD大鼠血清和健康大鼠血清作为一抗进行蛋白质印迹(Western blotting)分析,比较杂交蛋白点的差异,分析弓形虫速殖子特异性抗原。弓形虫速殖子总蛋白双向电泳考马斯亮蓝染色图谱(7cm×8cm,pH3～10)共检测到209个蛋白质斑点。Western blotting显示实验组抗原抗体反应点17个,对照组仅2个非特异性的蛋白结合点。
The total protein of Toxoplasma gondii RH strain tachyzoites was separated by two-dimensional electrophoresis （2-DE）, and Western blotting was performed to find out distinct antigens. 209 spots were detected through Coomassie brilliant blue-stained small gels （7×8 cm, pH 3-10）. Western blotting showed 17 specific antigen spots with sera of the experimental group and two nonspecific spots with the control sera.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2008年第4期318-320,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
贵州省科学技术基金(No.20043084)
贵州省高层次人才特助经费~~
关键词
弓形虫
速殖子
SD大鼠
抗原
双向电泳
蛋白质印迹
Toxoplasma gondii
Tachyzoite
SD rat
Antigen
Two-dimensional electrophoresis
Western blotting
